Multiple decrement life tables of Cephus cinctus Norton (Hymenoptera: Cephidae) across a set of barley cultivars: The importance of plant defense versus cannibalism

• Published in: PloS one Vol. 15; no. 9; p. e0238527
• Main Authors: Achhami, Buddhi B, Peterson, Robert K. D, Sherman, Jamie D, Reddy, Gadi V. P, Weaver, David K
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 11.09.2020; Public Library of Science (PLoS)
• Subjects: Animal behavior; Antibiosis; Barley; Biology and Life Sciences; Botanical research; Breeding; Cadavers; Cannibalism; Cephus cinctus; Control; Cultivars; Defense mechanisms; Diseases and pests; Ecology and Environmental Sciences; Elongation; Environmental science; Health aspects; Herbivores; Insect-plant relationships; Insects; Larvae; Life tables; Mortality; Mortality factors; Natural history; Oviposition; Parasitism; Pathogens; Physiological aspects; Plant breeding; Plant defenses; Plant resistance; Sawflies; Stems; Tables (data); Traditions; Wheat; United States; United States > US; Montana
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0238527

Accurately estimating cause-specific mortality for immature insect herbivores is usually difficult. The insects are exposed to abiotic and biotic mortality factors, causing cadavers to simply disappear before cause of mortality can be recorded. Also, insect herbivores are often highly mobile on hosts, making it difficult to follow patterns for individuals through time. In contrast, the wheat stem sawfly, Cephus cinctus Norton, spends its entire egg, larval, and pupal period inside a host stem. Therefore, with periodic sampling stage-specific causes of mortality can be ascertained. Consequently, we examined C. cinctus mortality in eight barley, Hordeum vulgare L., cultivars in two locations in Montana from 2016 to 2018 by collecting stem samples from stem elongation to crop maturity at weekly intervals, and collecting overwintered barley stubs the following spring and summer from the same plots. If larvae were present, we examined larval status-dead or alive-and categorized dead individuals into one of 5 mortality categories: plant defense, cannibalism, parasitism, pathogens, and unknown factors. We used multiple decrement life tables to estimate cause-specific mortality and irreplaceable mortality (the proportion of mortality from a given cause that cannot be replaced by other causes of mortality). Plant defense (antibiosis) caused 85.7 ± 3.6%, cannibalism (governed by antixenosis) caused 70.1 ± 7.6%, parasitism caused 13.8 ± 5.9%, unknown factors caused 38.5 ± 7.6%, and pathogens caused 14.7 ± 8.5% mortality in the presence of all causes of mortality. Similarly, irreplaceable mortality due to plant defense was 22.3 ± 6.4%, cannibalism was 29.1± 4.2%, unknown factors was 6.2 ± 1.8%, pathogens was 0.9 ± 0.5%, and parasitism was 1. 5 ± 0. 6%. Antibiosis traits primarily killed newly emerged larvae, while other traits supported more favorable oviposition decisions by females, increasing mortality by obligate cannibalism. Our results suggest that breeding barley for resistance to C. cinctus targeting both categories of traits (antibiosis and antixenosis) is a highly valuable tactic for management of this important pest.