Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones

• Published in: Biotechnology and bioengineering Vol. 99; no. 3; pp. 578 - 587
• Main Authors: Sleiman, Robert J, Gray, Peter P, McCall, Martin N, Codamo, Joe, Sunstrom, Noelle-Ann S
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.02.2008; Wiley; Wiley Subscription Services, Inc
• Subjects: Animal cells; Animals; Antibodies, Monoclonal - isolation & purification; Antibodies, Monoclonal - metabolism; Biological and medical sciences; Biotechnology; Cell Culture Techniques - methods; Cell Separation - methods; Cellular biology; CHO; CHO Cells; Cloning; Cloning, Molecular - methods; Cricetinae; Cricetulus; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Flow Cytometry - methods; Fluorescence; fluorescence activated cell sorting; Fundamental and applied biological sciences. Psychology; GFP; Methods. Procedures. Technologies; Microscopy, Fluorescence, Multiphoton - methods; Monoclonal antibodies; Pharmaceuticals; recombinant antibody; Rodents; selection; Antibody; Selection; Fluorescence; Cell sorting; Method; CHO; CHO cell line; GFP; Cell line; Green fluorescent protein; Recombinant protein; recombinant antibody; fluorescence activated cell sorting

The success of engineered monoclonal antibodies as biopharmaceuticals has generated considerable interest in strategies designed to accelerate development of antibody expressing cell lines. Stable mammalian cell lines that express therapeutic antibodies at high levels typically take 6-12 months to d...