Cryopreservation of 13 Commercial Cannabis sativa Genotypes Using In Vitro Nodal Explants

• Published in: Plants (Basel) Vol. 10; no. 9; p. 1794
• Main Authors: Downey, Cassandra D, Golenia, Gregory, Boudko, Ekaterina A, Jones, Andrew Maxwell P
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.08.2021; MDPI
• Subjects: Accuracy; Cannabinoids; Cannabis; Cannabis sativa; Cell culture; Cell division; conventional vitrification; Cryogenic temperature; Cryopreservation; droplet vitrification; Experiments; Explants; Fidelity; Genetic diversity; Genetic variability; Genetics; Genotypes; Germplasm; germplasm preservation; Incubation; Liquid nitrogen; Marijuana; Mutation; Nodes; Phenotypes; Product quality; Propagation; Regrowth; Shoots; tissue culture; Unloading; Vitrification; Canada; United States > US; conventional vitrification; Cannabis sativa; droplet vitrification; germplasm preservation; tissue culture

Cannabis has developed into a multi-billion-dollar industry that relies on clonal propagation of elite genetics with desirable agronomic and chemical phenotypes. While the goal of clonal propagation is to produce genetically uniform plants, somatic mutations can accumulate during growth and compromi...