Detection of Highly Poisonous Nerium oleander Using Quantitative Real-Time PCR with Specific Primers

• Published in: Toxins Vol. 14; no. 11; p. 776
• Main Authors: Bai, Xuanjiao, Wang, Gang, Ren, Ying, Han, Jianping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.11.2022; MDPI
• Subjects: Animals; Chromatography; Criminal investigations; DNA Primers - genetics; Food; Food contamination; Food contamination & poisoning; Food poisoning; Forensic science; Genetic testing; Humans; Identification; Identification methods; Ingestion; Livestock; Mixtures; Morphology; Nerium; Nerium - genetics; Nerium oleander; Plants, Toxic; Poisoning; Poisonous species; Poisons; Polymerase chain reaction; Primers; qPCR; Real time; Real-Time Polymerase Chain Reaction; Severe acute respiratory syndrome coronavirus 2; simulated forensic specimens; specific primers; Vegetables; qPCR; simulated forensic specimens; specific primers; Nerium oleander; food poisoning

is one of the most poisonous plants, and its accidental ingestion has frequently occurred in humans and livestock. It is vital to develop a rapid and accurate identification method for the timely rescue of oleander-poisoned patients and the investigation of poisoning cases. In this study, a specific...