Silencing of FAD synthase gene in Caenorhabditis elegans upsets protein homeostasis and impacts on complex behavioral patterns

• Published in: Biochimica et biophysica acta Vol. 1820; no. 4; pp. 521 - 531
• Main Authors: Liuzzi, Vania C., Giancaspero, Teresa A., Gianazza, Elisabetta, Banfi, Cristina, Barile, Maria, De Giorgi, Carla
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2012
• Subjects: adenosine triphosphate; alternative splicing; Amino Acid Sequence; animal models; Animals; Base Sequence; Behavior, Animal; biogenesis; biosynthesis; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; energy; enzyme activity; enzymes; FAD synthase; Flavin cofactors; Flavin-Adenine Dinucleotide - metabolism; flavoproteins; Flavoproteins - metabolism; Gene Silencing; genes; Genes, Helminth; Homeostasis; Humans; Locomotion; Mitochondria; Mitochondria - metabolism; Molecular Sequence Data; Nucleotidyltransferases - chemistry; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; oxygen consumption; polarography; protein composition; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - metabolism; proteomics; R53.1 gene; Reactive Oxygen Species - metabolism; reverse transcriptase polymerase chain reaction; RNA Interference; RNA, Small Interfering; spectroscopy; superoxide dismutase; Superoxide Dismutase - metabolism; Trans-splicing; two-dimensional gel electrophoresis; RNAi; Trans-splicing; FMN; TCA cycle; ALS; FAD; OXPHOS; SOD; FADS; FCCP; GR; RR-MADD; Flavin cofactors; Caenorhabditis elegans; Mitochondria; Rf; NGM; RFK; SDH; IPTG; FAD synthase; R53.1 gene; FUM

FAD synthase is a ubiquitous enzyme that catalyses the last step of FAD biosynthesis, allowing for the biogenesis of several flavoproteins. In humans different isoforms are generated by alternative splicing, isoform 1 being localized in mitochondria. Homology searching in Caenorabditis elegans leads...