Mitochondrial Fission Governed by Drp1 Regulates Exogenous Fatty Acid Usage and Storage in Hela Cells

In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mi...

Full description

Saved in:
Bibliographic Details
Published inMetabolites Vol. 11; no. 5; p. 322
Main Authors Song, Jae-Eun, Alves, Tiago C., Stutz, Bernardo, Šestan-Peša, Matija, Kilian, Nicole, Jin, Sungho, Diano, Sabrina, Kibbey, Richard G., Horvath, Tamas L.
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 18.05.2021
MDPI
Subjects
Bioenergetics
Carnitine palmitoyltransferase
Cell cycle
Cytology
Dynamin
fatty acid oxidation
Fatty acids
Glucose
Homeostasis
Kinases
lipid homeostasis
Lipids
Metabolism
Mitochondria
mitochondrial dynamics
Morphology
Oxidation
Palmitoyltransferase
Proteins
Respiration
lipid homeostasis
fatty acid oxidation
mitochondrial dynamics
Online AccessGet full text

Cover

Abstract In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mitochondrial morphology and lipid droplet accumulation in response to high exogenous fatty acids. We inhibited mitochondrial fission by silencing dynamin-related protein 1(DRP1) and observed the shift in fatty acid storage-usage balance. Inhibition of mitochondrial fission resulted in an increase in fatty acid content of lipid droplets and a decrease in mitochondrial fatty acid oxidation. Next, we overexpressed carnitine palmitoyltransferase-1 (CPT1), a key mitochondrial protein in fatty acid oxidation, to further examine the relationship between mitochondrial fatty acid usage and mitochondrial morphology. Mitochondrial fission plays a role in distributing exogenous fatty acids. CPT1A controlled the respiratory rate of mitochondrial fatty acid oxidation but did not cause a shift in the distribution of fatty acids between mitochondria and lipid droplets. Our data reveals a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning.
AbstractList In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mitochondrial morphology and lipid droplet accumulation in response to high exogenous fatty acids. We inhibited mitochondrial fission by silencing dynamin-related protein 1(DRP1) and observed the shift in fatty acid storage-usage balance. Inhibition of mitochondrial fission resulted in an increase in fatty acid content of lipid droplets and a decrease in mitochondrial fatty acid oxidation. Next, we overexpressed carnitine palmitoyltransferase-1 (CPT1), a key mitochondrial protein in fatty acid oxidation, to further examine the relationship between mitochondrial fatty acid usage and mitochondrial morphology. Mitochondrial fission plays a role in distributing exogenous fatty acids. CPT1A controlled the respiratory rate of mitochondrial fatty acid oxidation but did not cause a shift in the distribution of fatty acids between mitochondria and lipid droplets. Our data reveals a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning.
In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mitochondrial morphology and lipid droplet accumulation in response to high exogenous fatty acids. We inhibited mitochondrial fission by silencing dynamin-related protein 1(DRP1) and observed the shift in fatty acid storage-usage balance. Inhibition of mitochondrial fission resulted in an increase in fatty acid content of lipid droplets and a decrease in mitochondrial fatty acid oxidation. Next, we overexpressed carnitine palmitoyltransferase-1 (CPT1), a key mitochondrial protein in fatty acid oxidation, to further examine the relationship between mitochondrial fatty acid usage and mitochondrial morphology. Mitochondrial fission plays a role in distributing exogenous fatty acids. CPT1A controlled the respiratory rate of mitochondrial fatty acid oxidation but did not cause a shift in the distribution of fatty acids between mitochondria and lipid droplets. Our data reveals a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning.In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mitochondrial morphology and lipid droplet accumulation in response to high exogenous fatty acids. We inhibited mitochondrial fission by silencing dynamin-related protein 1(DRP1) and observed the shift in fatty acid storage-usage balance. Inhibition of mitochondrial fission resulted in an increase in fatty acid content of lipid droplets and a decrease in mitochondrial fatty acid oxidation. Next, we overexpressed carnitine palmitoyltransferase-1 (CPT1), a key mitochondrial protein in fatty acid oxidation, to further examine the relationship between mitochondrial fatty acid usage and mitochondrial morphology. Mitochondrial fission plays a role in distributing exogenous fatty acids. CPT1A controlled the respiratory rate of mitochondrial fatty acid oxidation but did not cause a shift in the distribution of fatty acids between mitochondria and lipid droplets. Our data reveals a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning.
Author Šestan-Peša, Matija
Kilian, Nicole
Jin, Sungho
Song, Jae-Eun
Alves, Tiago C.
Horvath, Tamas L.
Stutz, Bernardo
Kibbey, Richard G.
Diano, Sabrina
AuthorAffiliation 1 Yale Program in Integrative Cell Signaling and Neurobiology of Metabolism, Department of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06511, USA; jaeeun.song@yale.edu (J.-E.S.); bernardo.stutz@yale.edu (B.S.); matija.sestan-pesa@yale.edu (M.Š.-P.)
2 Laboratory Medicine, Institute for Clinical Chemistry, Technische Universität Dresden, 01069 Dresden, Germany; tiago.alves@uniklinikum-dresden.de
7 Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06511, USA
6 Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA; Richard.kibbey@yale.edu
8 Department of Ob/Gyn & Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06511, USA
3 Centre for Infectious Diseases, Parasitology, Heidelberg University Hospital, 69120 Heidelberg, Germany; nicole.kilian@med.uni-heidelberg.de
5 Department of Molecular Pharmacology and Therapeutics, Columbia University Irving Medical Center, New York, NY 1003
AuthorAffiliation_xml – name: 8 Department of Ob/Gyn & Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06511, USA
– name: 3 Centre for Infectious Diseases, Parasitology, Heidelberg University Hospital, 69120 Heidelberg, Germany; nicole.kilian@med.uni-heidelberg.de
– name: 4 Institute of Human Nutrition, Columbia University Irving Medical Center, New York, NY 10032, USA; sj3094@cumc.columbia.edu (S.J.); sd3449@cumc.columbia.edu (S.D.)
– name: 1 Yale Program in Integrative Cell Signaling and Neurobiology of Metabolism, Department of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06511, USA; jaeeun.song@yale.edu (J.-E.S.); bernardo.stutz@yale.edu (B.S.); matija.sestan-pesa@yale.edu (M.Š.-P.)
– name: 5 Department of Molecular Pharmacology and Therapeutics, Columbia University Irving Medical Center, New York, NY 10032, USA
– name: 6 Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA; Richard.kibbey@yale.edu
– name: 7 Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06511, USA
– name: 2 Laboratory Medicine, Institute for Clinical Chemistry, Technische Universität Dresden, 01069 Dresden, Germany; tiago.alves@uniklinikum-dresden.de
Author_xml – sequence: 1
  givenname: Jae-Eun
  surname: Song
  fullname: Song, Jae-Eun
– sequence: 2
  givenname: Tiago C.
  surname: Alves
  fullname: Alves, Tiago C.
– sequence: 3
  givenname: Bernardo
  orcidid: 0000-0001-5282-6400
  surname: Stutz
  fullname: Stutz, Bernardo
– sequence: 4
  givenname: Matija
  orcidid: 0000-0002-9873-6387
  surname: Šestan-Peša
  fullname: Šestan-Peša, Matija
– sequence: 5
  givenname: Nicole
  surname: Kilian
  fullname: Kilian, Nicole
– sequence: 6
  givenname: Sungho
  surname: Jin
  fullname: Jin, Sungho
– sequence: 7
  givenname: Sabrina
  surname: Diano
  fullname: Diano, Sabrina
– sequence: 8
  givenname: Richard G.
  surname: Kibbey
  fullname: Kibbey, Richard G.
– sequence: 9
  givenname: Tamas L.
  surname: Horvath
  fullname: Horvath, Tamas L.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34069800$$D View this record in MEDLINE/PubMed
BookMark eNp1ks1PHCEYxkljU6312mND0ksva_kaBi5NzOqqiU2Ttp4JM_POyGYWtsCY7n9f1lXjmpQLBH7P837wvkcHPnhA6CMlp5xr8nUF2TaBUlIRztgbdMQYVTOqlT54cT5EJyktSVmSVDWh79AhF0RqRcgRgu8uh_Yu-C46O-KFS8kFjy_DPUQPHW42-DyuKf4JwzTaDAlf_A0D-DAlvLA5b_BZ6zp8m-wA2PoO_8ohbs_O4ysYLZ7DOKYP6G1vxwQnj_sxul1c_J5fzW5-XF7Pz25mrVAiz9peaU44UF2DkkLpmitJlai0VBYoU72oGip1TWUR1LqvFbAGtOYdlxXl_Bhd73y7YJdmHd3Kxo0J1pmHixAHY2N27Qim1oyRmlLJqm3wztpGM61YCSrA1rR4fdt5radmBV0LPkc77pnuv3h3Z4ZwbxStaqZYMfjyaBDDnwlSNiuX2tIO66G0z7CKSyFLiaKgn1-hyzBFX1q1pRjVQjFZqE8vM3pO5ek3C3C6A9oYUorQPyOUmO3EmP2JKQLxStC6bHOZgFKRG_8n-wcRXMJX
CitedBy_id crossref_primary_10_3389_fendo_2023_1267170
crossref_primary_10_3390_nu15092222
crossref_primary_10_3390_ijms24010629
crossref_primary_10_1007_s00018_022_04401_3
crossref_primary_10_1038_s41418_022_00974_5
crossref_primary_10_15252_embj_2022111901
crossref_primary_10_1002_cac2_12247
crossref_primary_10_1083_jcb_202401024
crossref_primary_10_1038_s41598_025_94652_4
crossref_primary_10_1016_j_devcel_2024_09_004
crossref_primary_10_1038_s41374_021_00724_0
crossref_primary_10_1186_s12974_024_03154_z
crossref_primary_10_2147_JIR_S340420
crossref_primary_10_3389_fnagi_2022_930105
crossref_primary_10_1016_j_jff_2022_105125
crossref_primary_10_1016_j_molcel_2024_06_009
crossref_primary_10_1073_pnas_2119824119
crossref_primary_10_1126_sciadv_abj2814
crossref_primary_10_1177_25152564241261228
Cites_doi 10.7554/eLife.64351
10.1016/j.celrep.2019.06.058
10.1038/ncb2220
10.1016/j.cmet.2013.03.002
10.1016/j.cell.2013.09.003
10.1016/S0006-291X(03)00050-0
10.1038/s41592-018-0145-5
10.1016/j.cmet.2006.12.002
10.2337/db07-1781
10.1073/pnas.1107402108
10.3389/fphys.2017.00902
10.1074/jbc.M109.050443
10.1074/jbc.R200006200
10.1038/s42255-020-0245-2
10.1042/bj3340233
10.1016/j.cmet.2015.08.021
10.3389/fphys.2014.00282
10.1016/j.devcel.2015.01.029
10.1016/j.molcel.2020.10.013
10.1016/j.cell.2013.09.004
10.1016/j.nut.2006.04.008
10.1016/j.cell.2016.05.035
10.1074/jbc.M211761200
10.1074/jbc.M111.228692
10.1038/nature07181
10.1371/journal.pone.0000107
10.1126/science.aab4138
10.1194/jlr.M017939
10.1016/j.cmet.2018.03.003
10.1016/bs.ircmb.2017.12.007
10.1016/S0021-9258(17)34693-8
10.1194/jlr.R025882
10.1038/nrendo.2014.160
10.1016/j.devcel.2017.06.003
10.1126/science.1219855
10.1016/j.bbrc.2005.10.016
ContentType Journal Article
Copyright 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2021 by the authors. 2021
Copyright_xml – notice: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2021 by the authors. 2021
DBID AAYXX
CITATION
NPM
7QR
8FD
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PHGZM
PHGZT
PIMPY
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.3390/metabo11050322
DatabaseName CrossRef
PubMed
Chemoreception Abstracts
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
Biological Science Database
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
PubMed
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
ProQuest One Applied & Life Sciences
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
Chemoreception Abstracts
ProQuest Central (New)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Biological Science Database
ProQuest SciTech Collection
Biotechnology and BioEngineering Abstracts
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
ProQuest One Academic (New)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database

MEDLINE - Academic
PubMed
CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: ProQuest Central Database Suite (ProQuest)
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
EISSN 2218-1989
ExternalDocumentID oai_doaj_org_article_79220711625c484daab929828974ea71
PMC8157282
34069800
10_3390_metabo11050322
Genre Journal Article
GrantInformation_xml – fundername: NIDDK NIH HHS
  grantid: R01 DK110181
– fundername: NIH HHS
  grantid: AG052005, AG052986, AG051459, DK111178, DK045735, DK097566, DK110181, DK045735-26
– fundername: NIDDK NIH HHS
  grantid: P30 DK045735
– fundername: NIDDK NIH HHS
  grantid: R01 DK127637
– fundername: Hungarian National Research, Development and Innovation Office
  grantid: NKFI-KPP-126998
GroupedDBID 53G
5VS
8FE
8FH
AADQD
AAFWJ
AAYXX
AFKRA
AFPKN
AFZYC
ALMA_UNASSIGNED_HOLDINGS
BBNVY
BENPR
BHPHI
CCPQU
CITATION
DIK
GROUPED_DOAJ
HCIFZ
HYE
IAO
KQ8
LK8
M48
M7P
MODMG
M~E
OK1
PGMZT
PHGZM
PHGZT
PIMPY
PROAC
RPM
NPM
PQGLB
7QR
8FD
ABUWG
AZQEC
DWQXO
FR3
GNUQQ
P64
PKEHL
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
PUEGO
ID FETCH-LOGICAL-c484t-cf89303e197e8648973861845968ae128f45b169716c4879f78e2be993d365133
IEDL.DBID M48
ISSN 2218-1989
IngestDate Wed Aug 27 01:27:25 EDT 2025
Thu Aug 21 18:16:01 EDT 2025
Fri Jul 11 08:36:50 EDT 2025
Fri Jul 25 11:55:24 EDT 2025
Mon Jul 21 06:02:27 EDT 2025
Thu Apr 24 22:56:46 EDT 2025
Tue Jul 01 00:44:04 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 5
Keywords lipid homeostasis
fatty acid oxidation
mitochondrial dynamics
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c484t-cf89303e197e8648973861845968ae128f45b169716c4879f78e2be993d365133
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0001-5282-6400
0000-0002-9873-6387
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.3390/metabo11050322
PMID 34069800
PQID 2532194826
PQPubID 2032362
ParticipantIDs doaj_primary_oai_doaj_org_article_79220711625c484daab929828974ea71
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8157282
proquest_miscellaneous_2536467384
proquest_journals_2532194826
pubmed_primary_34069800
crossref_primary_10_3390_metabo11050322
crossref_citationtrail_10_3390_metabo11050322
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20210518
PublicationDateYYYYMMDD 2021-05-18
PublicationDate_xml – month: 5
  year: 2021
  text: 20210518
  day: 18
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Metabolites
PublicationTitleAlternate Metabolites
PublicationYear 2021
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Alam (ref_11) 1998; 334
Koss (ref_28) 2005; 338
Nasrallah (ref_6) 2014; 10
Kilian (ref_36) 2018; 15
Dietrich (ref_16) 2013; 155
Garbarino (ref_33) 2009; 284
Adachi (ref_27) 2020; 80
Wang (ref_20) 2011; 52
ref_18
Coppola (ref_14) 2007; 5
Schneeberger (ref_17) 2013; 155
Srivastava (ref_25) 2012; 53
Pinkosky (ref_24) 2020; 2
Rambold (ref_9) 2011; 108
Lee (ref_29) 2011; 286
Youle (ref_4) 2012; 337
Andrews (ref_15) 2008; 454
Alves (ref_21) 2015; 22
Rambold (ref_8) 2015; 32
Cohen (ref_19) 2018; 337
Benador (ref_22) 2018; 27
Jesinkey (ref_30) 2019; 28
Gomes (ref_35) 2011; 13
Ishihara (ref_1) 2003; 301
Nguyen (ref_23) 2017; 42
Molina (ref_5) 2009; 58
Carta (ref_10) 2017; 8
Liesa (ref_7) 2013; 17
Koch (ref_26) 2003; 278
Aon (ref_32) 2014; 5
Buck (ref_2) 2016; 166
Ortega (ref_3) 2006; 1
Owen (ref_31) 2002; 277
McGarry (ref_12) 1978; 253
Finn (ref_34) 2006; 22
Toyama (ref_13) 2016; 351
References_xml – ident: ref_18
  doi: 10.7554/eLife.64351
– volume: 28
  start-page: 759
  year: 2019
  ident: ref_30
  article-title: Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2019.06.058
– volume: 13
  start-page: 589
  year: 2011
  ident: ref_35
  article-title: During autophagy mitochondria elongate, are spared from degradation and sustain cell viability
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb2220
– volume: 17
  start-page: 491
  year: 2013
  ident: ref_7
  article-title: Mitochondrial Dynamics in the Regulation of Nutrient Utilization and Energy Expenditure
  publication-title: Cell Metab.
  doi: 10.1016/j.cmet.2013.03.002
– volume: 155
  start-page: 172
  year: 2013
  ident: ref_17
  article-title: Mitofusin 2 in POMC Neurons Connects ER Stress with Leptin Resistance and Energy Imbalance
  publication-title: Cell
  doi: 10.1016/j.cell.2013.09.003
– volume: 301
  start-page: 891
  year: 2003
  ident: ref_1
  article-title: Regulation of mitochondrial morphology by membrane potential, and DRP1-dependent division and FZO1-dependent fusion reaction in mammalian cells
  publication-title: Biochem. Biophys. Res. Commun.
  doi: 10.1016/S0006-291X(03)00050-0
– volume: 15
  start-page: 755
  year: 2018
  ident: ref_36
  article-title: Assessing photodamage in live-cell STED microscopy
  publication-title: Nat. Methods
  doi: 10.1038/s41592-018-0145-5
– volume: 5
  start-page: 21
  year: 2007
  ident: ref_14
  article-title: A Central Thermogenic-like Mechanism in Feeding Regulation: An Interplay between Arcuate Nucleus T3 and UCP2
  publication-title: Cell Metab.
  doi: 10.1016/j.cmet.2006.12.002
– volume: 58
  start-page: 2303
  year: 2009
  ident: ref_5
  article-title: Mitochondrial Networking Protects-Cells From Nutrient-Induced Apoptosis
  publication-title: Diabetes
  doi: 10.2337/db07-1781
– volume: 108
  start-page: 10190
  year: 2011
  ident: ref_9
  article-title: Tubular network formation protects mitochondria from autophagosomal degradation during nutrient starvation
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1107402108
– volume: 8
  start-page: 902
  year: 2017
  ident: ref_10
  article-title: Palmitic acid: Physiological role, metabolism and nutritional implications
  publication-title: Front. Physiol.
  doi: 10.3389/fphys.2017.00902
– volume: 284
  start-page: 30994
  year: 2009
  ident: ref_33
  article-title: Sterol and Diacylglycerol Acyltransferase Deficiency Triggers Fatty Acid-mediated Cell Death
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M109.050443
– volume: 277
  start-page: 30409
  year: 2002
  ident: ref_31
  article-title: The key role of anaplerosis and cataplerosis for citric acid cycle function
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.R200006200
– volume: 2
  start-page: 873
  year: 2020
  ident: ref_24
  article-title: Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms
  publication-title: Nat. Metab.
  doi: 10.1038/s42255-020-0245-2
– volume: 334
  start-page: 233
  year: 1998
  ident: ref_11
  article-title: Malonyl-CoA and the regulation of fatty acid oxidation in soleus muscle
  publication-title: Biochem. J.
  doi: 10.1042/bj3340233
– volume: 22
  start-page: 936
  year: 2015
  ident: ref_21
  article-title: Integrated, Step-Wise, Mass-Isotopomeric Flux Analysis of the TCA Cycle
  publication-title: Cell Metab.
  doi: 10.1016/j.cmet.2015.08.021
– volume: 5
  start-page: 282
  year: 2014
  ident: ref_32
  article-title: Mitochondrial and cellular mechanisms for managing lipid excess
  publication-title: Front. Physiol.
  doi: 10.3389/fphys.2014.00282
– volume: 32
  start-page: 678
  year: 2015
  ident: ref_8
  article-title: Fatty Acid Trafficking in Starved Cells: Regulation by Lipid Droplet Lipolysis, Autophagy, and Mitochondrial Fusion Dynamics
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2015.01.029
– volume: 80
  start-page: 621
  year: 2020
  ident: ref_27
  article-title: Drp1 Tubulates the ER in a GTPase-Independent Manner
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2020.10.013
– volume: 155
  start-page: 188
  year: 2013
  ident: ref_16
  article-title: Mitochondrial dynamics controlled by mitofusins regulate agrp neuronal activity and diet-induced obesity
  publication-title: Cell
  doi: 10.1016/j.cell.2013.09.004
– volume: 22
  start-page: 830
  year: 2006
  ident: ref_34
  article-title: Proteolytic and lipolytic responses to starvation
  publication-title: Nutrition
  doi: 10.1016/j.nut.2006.04.008
– volume: 166
  start-page: 1
  year: 2016
  ident: ref_2
  article-title: Mitochondrial Dynamics Controls T Cell Fate through Article Mitochondrial Dynamics Controls T Cell Fate through Metabolic Programming
  publication-title: Cell
  doi: 10.1016/j.cell.2016.05.035
– volume: 278
  start-page: 8597
  year: 2003
  ident: ref_26
  article-title: Dynamin-like Protein 1 Is Involved in Peroxisomal Fission
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M211761200
– volume: 286
  start-page: 25655
  year: 2011
  ident: ref_29
  article-title: Mitochondrial carnitine palmitoyltransferase 1a (CPT1a) is part of an outer membrane fatty acid transfer complex
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M111.228692
– volume: 454
  start-page: 846
  year: 2008
  ident: ref_15
  article-title: UCP2 mediates ghrelin’s action on NPY/AgRP neurons by lowering free radicals
  publication-title: Nature
  doi: 10.1038/nature07181
– volume: 1
  start-page: e107
  year: 2006
  ident: ref_3
  article-title: Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0000107
– volume: 351
  start-page: 275
  year: 2016
  ident: ref_13
  article-title: AMP-activated protein kinase mediates mitochondrial fission in response to energy stress
  publication-title: Science
  doi: 10.1126/science.aab4138
– volume: 52
  start-page: 2159
  year: 2011
  ident: ref_20
  article-title: Perilipin 5, a lipid droplet-associated protein, provides physical and metabolic linkage to mitochondria
  publication-title: J. Lipid Res.
  doi: 10.1194/jlr.M017939
– volume: 27
  start-page: 869
  year: 2018
  ident: ref_22
  article-title: Mitochondria Bound to Lipid Droplets Have Unique Bioenergetics, Composition, and Dynamics that Support Lipid Droplet Expansion
  publication-title: Cell Metab.
  doi: 10.1016/j.cmet.2018.03.003
– volume: 337
  start-page: 83
  year: 2018
  ident: ref_19
  article-title: Lipid Droplets as Organelles
  publication-title: Int. Rev. Cell Mol. Biol.
  doi: 10.1016/bs.ircmb.2017.12.007
– volume: 253
  start-page: 4128
  year: 1978
  ident: ref_12
  article-title: Carnitine palmitoyltransferase I. The site of inhibition of hepatic fatty acid oxidation by malonyl-CoA
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(17)34693-8
– volume: 53
  start-page: 2490
  year: 2012
  ident: ref_25
  article-title: AMP-activated protein kinase: An emerging drug target to regulate imbalances in lipid and carbohydrate metabolism to treat cardio-metabolic diseases: Thematic Review Series: New Lipid and Lipoprotein Targets for the Treatment of Cardiometabolic Diseases
  publication-title: J. Lipid Res.
  doi: 10.1194/jlr.R025882
– volume: 10
  start-page: 650
  year: 2014
  ident: ref_6
  article-title: Mitochondrial dynamics in the central regulation of metabolism
  publication-title: Nat. Rev. Endocrinol.
  doi: 10.1038/nrendo.2014.160
– volume: 42
  start-page: 9
  year: 2017
  ident: ref_23
  article-title: DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2017.06.003
– volume: 337
  start-page: 1062
  year: 2012
  ident: ref_4
  article-title: Mitochondrial fission, fusion, and stress
  publication-title: Science
  doi: 10.1126/science.1219855
– volume: 338
  start-page: 757
  year: 2005
  ident: ref_28
  article-title: CPT1α over-expression increases long-chain fatty acid oxidation and reduces cell viability with incremental palmitic acid concentration in 293T cells
  publication-title: Biochem. Biophys. Res. Commun.
  doi: 10.1016/j.bbrc.2005.10.016
SSID ssj0000605701
Score 2.3058922
Snippet In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 322
SubjectTerms Bioenergetics
Carnitine palmitoyltransferase
Cell cycle
Cytology
Dynamin
fatty acid oxidation
Fatty acids
Glucose
Homeostasis
Kinases
lipid homeostasis
Lipids
Metabolism
Mitochondria
mitochondrial dynamics
Morphology
Oxidation
Palmitoyltransferase
Proteins
Respiration
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LT9wwELYQp14QhT7SQuVKVXuKSPyIneOWskKV4EC7ErfIiSdipcUgNpXYf89MHFYbRMWFW5RMImdm7JkvGX_D2LfS1KJsRZl6JU2qTFukdWERtZYAsvXCNBntHT47L05n6velvtxo9UU1YZEeOCruyJRCYBjMMU9vlFXeuRojOuEEo8D1u8cFxrwNMBXXYMxDsjyyNErE9UfX0KFWMdjpTAoxikI9Wf9zGebTQsmNyDPdZTtDysgncahv2RaEPbY_CQiXr1f8O--LOPuv4_sMznCG4ooWPDkWn86pyDXw2FEXPK9X_Nfdbc4vYgt6WPKT-5vI08qnrutWfNLMPZ9RuRl3wfM_iMnpeB44RijHj2GxWL5js-nJ3-PTdOikkJLOurRpMS3JJOSlAVso1J201OlFl4V1gCGqVbrOC6KTwhtM2RoLogbMXbwsqAPMe7YdbgJ8ZFzJ1kkhjTONV1pq57z1OgMvmgYfLhKWPmq2agaacep2sagQbpAlqrElEvZjLX8bCTb-K_mTDLWWImLs_gS6SzW4S_WSuyTs4NHM1TBbl5XQEhduhUgrYV_Xl3Ge0c8TFwBtQDKFohapKmEfolesRyJp-zBm3gkzI38ZDXV8Jcyvei5vm2uDqPfTa7zbZ_ZGUMUNccvaA7bd3f2DQ0yZuvpLPzseANU_EYs
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fb9MwED5B98ILGowfgTEZCcFTtMR2Yudp6rZWE9ImNKi0t8iJHVapc0ubSfS_312SBoKAtyhxIsd3Z3-fc_kO4EOmCp5VPAutFCqUqkrDItXIWjPnRGW5KiP6d_jyKr2Yyc83yU234bbp0ip3c2IzUdtlSXvkxzwRGFwS0fDJ6kdIVaPo62pXQuMx7OEUrPUI9k4nV1-u-12WCNG6iuJWrVEgvz--czWOLi56SSQ4H6xGjWj_35DmnwmTv61A03142kFHNm5t_QweOf8cDsYeafPdln1kTTJns0t-AO4SIxVnNm_Jwdh0TsmunrWVdZ1lxZadr1cxu25L0bsNm_xctnqtbGrqesvG5dyyGaWdMeMt-4rcnI7nnuFKZdiZWyw2L2A2nXw7uwi7igphKbWsw7JCeBIJF2fK6VTqjEp-IsdLslQbhyNYyaSIU5KVwhtUVinteOEQw1iRUiWYlzDyS-9eA5OiMoILZVRpZSISY6y2SeQsL0t8OA8g3I1sXnZy41T1YpEj7SBL5ENLBPCpb79qhTb-2fKUDNW3IoHs5sRy_T3v4i1XGeeInmKkd_Tq1pgCgSDRSyWdUXEAhzsz513UbvJfPhbA-_4yxht9RDHeoQ2oTSqpVKoM4FXrFX1PBP1GjAg8ADXwl0FXh1f8_LbR9NZxopD9vvl_t97CE045NaQeqw9hVK_v3TsERXVx1Hn-Ayi0DCM
  priority: 102
  providerName: ProQuest
Title Mitochondrial Fission Governed by Drp1 Regulates Exogenous Fatty Acid Usage and Storage in Hela Cells
URI https://www.ncbi.nlm.nih.gov/pubmed/34069800
https://www.proquest.com/docview/2532194826
https://www.proquest.com/docview/2536467384
https://pubmed.ncbi.nlm.nih.gov/PMC8157282
https://doaj.org/article/79220711625c484daab929828974ea71
Volume 11
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwdV3fb9MwED7BJqG9IGD8CBuVkRA8BRr_iJ0HhLrRakLqhAaV9hY5sQOVOne0mbT-99wlaVlR9xYlrpX47nL3pefvA3iX6YJnFc9iJ4WOpa7SuEgNotbMe1E5rss-7R0en6dnE_ntUl3-63_qFnC5E9qRntRkMft4-2f1BQP-MyFOhOyfrnyNC4Z5TPXRPR_CPmYlTWoG467Ub9_KWJk0asgcs1pMrUIth-OOKQ7gkaAdoYb2vd1JVw2r_65S9P-OyjspavQEHne1JRu0zvAUHvjwDA4HAXH11Yq9Z023Z_MZ_RD8GEMZnz448kA2mlI3bGCt9K53rFixr4vrhF20WvV-yYa385bQlY1sXa_YoJw6NqG-NGaDYz8QvNPxNDBMZZad-tls-Rwmo-HP07O4k1yIS2lkHZcV1i994ZNMe5NKk5EmKIJAlaXGesxllVRFkhLvFP5AZ5U2nhceixwnUpKKeQF7YR78K2BSVFZwoa0unVRCWeuMU33veFni5DyCeL2yednxkZMsxixHXEJGybeNEsGHzfjrlonj3pEnZKjNKGLQbk7MF7_yLiBznXGO5VWC-I8e3VlbYKVI-FNLb3USwfHazPnaK3OuBL7hJUKyCN5uLmNA0r8sNni0AY1JJWmpyghetl6xuZO1V0Wgt_xl61a3r4Tp74b02yRKIzx-fe-cR3DAqd-GmGXNMezVixv_BgumuujB_snw_PtFr_ng0Gvi4i8JdhJk
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6V9AAXBJSHS4FF4nGyau-uvfYBobRNlNImQqWRejNr77pESp00MYL8KX4jM7ZjCAJuvVn22lp7nt96dj6AV7FKeZzz2DVSKFeqPHTTMELUGlsrcsNV5tHe4eEoHIzlh4vgYgt-rPfCUFnl2idWjtrMMloj3-eBQOOSmA2_n1-7xBpFf1fXFBq1WpzY1TeEbMt3x0co39ec93vnhwO3YRVwMxnJ0s1yDNGesH6sbBTKKCbaS8Q5QRxG2qK7zmWQ-iG1VsIbVJyryPLUYhw3IiQ2FHzuLdiWIvR4B7YPeqOPZ-2qjofoQHl-3R1SiNjbv7IlShODbOAJzjeiX0US8LfM9s8Czd8iXv8e3G1SVdatdes-bNniAex0C4TpVyv2hlXFo9Wq_A7YIXoG9KSFIYVm_QkV1xasZvK1hqUrdrSY--zMXhJfmF2y3vdZ3R-W9XVZrlg3mxg2pjI3pgvDPpWom3g8KRhGRs0O7XS6fAjjG_nWj6BTzAr7BJgUuRZcKK0yIwMRaG0iE3jW8CzDh3MH3PWXTbKmvTmxbEwThDkkiWRTEg68bcfP68Ye_xx5QIJqR1FD7urEbHGZNPadqJhzzNZ8hJP06kbrFBNPgrNKWq18B_bWYk4aL7FMfum0Ay_by2jf9NNGFxZlQGNCSdSs0oHHtVa0MxG0bRkzfgfUhr5sTHXzSjH5UvUQj_xAIdre_f-0XsDtwfnwNDk9Hp08hTuc6nmoc220B51y8dU-w4SsTJ83VsDg800b3k9Z5kWc
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6VVEJcEFAepgUWicfJir279toHhNImUUtpVBUi9eauvesSKXXSxAjy1_h1zNiOIQi49WbZa2vteX7r2fkAXsUq5XHOY9dIoVyp8tBNwwhRa2ytyA1XmUd7h09G4eFYfjgPzrfgx3ovDJVVrn1i5ajNLKM18i4PBBqXxGy4mzdlEaf94fv5tUsMUvSndU2nUavIsV19Q_i2fHfUR1m_5nw4-Hxw6DYMA24mI1m6WY7h2hPWj5WNQhnFRIGJmCeIw0hbdN25DFI_pDZLeIOKcxVZnlqM6UaExIyCz70F24pQUQe29wej07N2hcdDpKA8v-4UKUTsda9siZLFgBt4gvONSFgRBvwty_2zWPO36De8B3ebtJX1aj27D1u2eAA7vQIh-9WKvWFVIWm1Qr8D9gS9BHrVwpBys-GECm0LVrP6WsPSFesv5j47s5fEHWaXbPB9VveKZUNdlivWyyaGjankjenCsE8l6ikeTwqGUVKzAzudLh_C-Ea-9SPoFLPCPgEmRa4FF0qrzMhABFqbyASeNTzL8OHcAXf9ZZOsaXVOjBvTBCEPSSLZlIQDb9vx87rJxz9H7pOg2lHUnLs6MVtcJo2tJyrmHBXCR2hJr260TjEJJWirpNXKd2BvLeak8RjL5Jd-O_CyvYy2Tj9wdGFRBjQmlETTKh14XGtFOxNBW5gx-3dAbejLxlQ3rxSTL1U_8cgPFCLvp_-f1gu4jQaXfDwaHe_CHU6lPdTENtqDTrn4ap9hblamzxsjYHBx03b3E5w3SdE
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Mitochondrial+Fission+Governed+by+Drp1+Regulates+Exogenous+Fatty+Acid+Usage+and+Storage+in+Hela+Cells&rft.jtitle=Metabolites&rft.au=Song%2C+Jae-Eun&rft.au=Alves%2C+Tiago+C&rft.au=Stutz%2C+Bernardo&rft.au=%C5%A0estan-Pe%C5%A1a%2C+Matija&rft.date=2021-05-18&rft.issn=2218-1989&rft.eissn=2218-1989&rft.volume=11&rft.issue=5&rft_id=info:doi/10.3390%2Fmetabo11050322&rft_id=info%3Apmid%2F34069800&rft.externalDocID=34069800
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2218-1989&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2218-1989&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2218-1989&client=summon