Proliferation and Tumorigenesis of a Murine Sarcoma Cell Line in the Absence of DICER1

• Published in: Cancer cell Vol. 21; no. 6; pp. 848 - 855
• Main Authors: Ravi, Arvind, Gurtan, Allan M., Kumar, Madhu S., Bhutkar, Arjun, Chin, Christine, Lu, Victoria, Lees, Jacqueline A., Jacks, Tyler, Sharp, Phillip A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.06.2012
• Subjects: Animals; Antineoplastic Agents, Hormonal - pharmacology; Blotting, Northern; Blotting, Western; Cell Line, Tumor; Cell Proliferation; Cell Transformation, Neoplastic - genetics; Cells, Cultured; DEAD-box RNA Helicases - deficiency; DEAD-box RNA Helicases - genetics; Flow Cytometry; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - drug effects; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; MicroRNAs - genetics; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III - deficiency; Ribonuclease III - genetics; Sarcoma - genetics; Sarcoma - metabolism; Sarcoma - pathology; Tamoxifen - pharmacology

MicroRNAs are a class of short ∼22 nucleotide RNAs predicted to regulate nearly half of all protein coding genes, including many involved in basal cellular processes and organismal development. Although a global reduction in miRNAs is commonly observed in various human tumors, complete loss has not...