Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly

• Published in: Toxins Vol. 11; no. 2; p. 71
• Main Authors: Nan, Mina, Bi, Yang, Xue, Huali, Xue, Sulin, Long, Haitao, Pu, Lumei, Fu, Guorui
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.01.2019; MDPI
• Subjects: Aptamers; Aptamers, Nucleotide - chemistry; Aspergillus; Biosensing Techniques; Chemical reactions; Chromatography; Commodities; Dielectric Spectroscopy; Electrochemical impedance spectroscopy; Electrochemistry; Electrodes; Food Contamination - analysis; Fruit and Vegetable Juices - analysis; Fruit juices; Fruits; Gold; Gold - chemistry; grape; Grapes; Inoculation; label-free; layer-by-layer self-assembly; Metal Nanoparticles - chemistry; Methods; Nanoparticles; Ochratoxin A; Ochratoxins - analysis; Ochratoxins - chemistry; Phenolic compounds; Phenols; Quantitation; Self-assembly; Sensors; Spectroscopy; thiolated aptamer; Vitis - chemistry; Vitis - microbiology; Wine - analysis; Wines; label-free; ochratoxin A; thiolated aptamer; grape; layer-by-layer self-assembly

A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the rel...