Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly

A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the rel...

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Published inToxins Vol. 11; no. 2; p. 71
Main Authors Nan, Mina, Bi, Yang, Xue, Huali, Xue, Sulin, Long, Haitao, Pu, Lumei, Fu, Guorui
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 28.01.2019
MDPI
Subjects
Aptamers
Aptamers, Nucleotide - chemistry
Aspergillus
Biosensing Techniques
Chemical reactions
Chromatography
Commodities
Dielectric Spectroscopy
Electrochemical impedance spectroscopy
Electrochemistry
Electrodes
Food Contamination - analysis
Fruit and Vegetable Juices - analysis
Fruit juices
Fruits
Gold
Gold - chemistry
grape
Grapes
Inoculation
label-free
layer-by-layer self-assembly
Metal Nanoparticles - chemistry
Methods
Nanoparticles
Ochratoxin A
Ochratoxins - analysis
Ochratoxins - chemistry
Phenolic compounds
Phenols
Quantitation
Self-assembly
Sensors
Spectroscopy
thiolated aptamer
Vitis - chemistry
Vitis - microbiology
Wine - analysis
Wines
label-free
ochratoxin A
thiolated aptamer
grape
layer-by-layer self-assembly
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Abstract A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%–104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
AbstractList A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔR ct ) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%–104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%–104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%⁻104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%⁻104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔR ) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%⁻104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%⁻104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
Author Pu, Lumei
Bi, Yang
Xue, Sulin
Xue, Huali
Nan, Mina
Fu, Guorui
Long, Haitao
AuthorAffiliation 1 College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; nanmn@gsau.edu.cn (M.N.); xuesulin@outlook.com (S.X.)
2 College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
3 College of Science, Gansu Agricultural University, Lanzhou 730070, China; longht@gsau.edu.cn (H.L.); pulm@gsau.edu.cn (L.P.); fugr@gsau.edu.cn (G.F.)
AuthorAffiliation_xml – name: 3 College of Science, Gansu Agricultural University, Lanzhou 730070, China; longht@gsau.edu.cn (H.L.); pulm@gsau.edu.cn (L.P.); fugr@gsau.edu.cn (G.F.)
– name: 2 College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
– name: 1 College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; nanmn@gsau.edu.cn (M.N.); xuesulin@outlook.com (S.X.)
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/30696025$$D View this record in MEDLINE/PubMed
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Keywords label-free
ochratoxin A
thiolated aptamer
grape
layer-by-layer self-assembly
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Snippet A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination...
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StartPage 71
SubjectTerms Aptamers
Aptamers, Nucleotide - chemistry
Aspergillus
Biosensing Techniques
Chemical reactions
Chromatography
Commodities
Dielectric Spectroscopy
Electrochemical impedance spectroscopy
Electrochemistry
Electrodes
Food Contamination - analysis
Fruit and Vegetable Juices - analysis
Fruit juices
Fruits
Gold
Gold - chemistry
grape
Grapes
Inoculation
label-free
layer-by-layer self-assembly
Metal Nanoparticles - chemistry
Methods
Nanoparticles
Ochratoxin A
Ochratoxins - analysis
Ochratoxins - chemistry
Phenolic compounds
Phenols
Quantitation
Self-assembly
Sensors
Spectroscopy
thiolated aptamer
Vitis - chemistry
Vitis - microbiology
Wine - analysis
Wines
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Title Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly
URI https://www.ncbi.nlm.nih.gov/pubmed/30696025
https://www.proquest.com/docview/2550289337
https://www.proquest.com/docview/2179453862
https://pubmed.ncbi.nlm.nih.gov/PMC6410112
https://doaj.org/article/94a02b4495d4422aa7597fbc4eba28dc
Volume 11
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