A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis

A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 172; no. 1; pp. 279 - 283
Main Authors Wadsworth, Gregory J., Redinbaugh, Margaret G., Scandalios, John G.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.07.1988
Elsevier
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blotting, Northern
Fundamental and applied biological sciences. Psychology
gene expression
mRNA
plant
Plants - genetics
RNA - isolation & purification
RNA blot analysis
RNA, Messenger - analysis
RNA, purification
Spectrophotometry, Ultraviolet
Transcription, Genetic
Zea mays - genetics
RNA blot analysis
mRNA
RNA, purification
gene expression
plant
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Summary:A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5 h. This method is well suited for the isolation of RNA from large numbers of samples or from samples of limited quantity.
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SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(88)90443-5