A Four-Monoclonal Antibody Combination Potently Neutralizes Multiple Botulinum Neurotoxin Serotypes C and D

• Published in: Toxins Vol. 13; no. 9; p. 641
• Main Authors: Garcia-Rodriguez, Consuelo, Yan, Shude, Geren, Isin N, Knopp, Kristeene A, Dong, Jianbo, Sun, Zhengda, Lou, Jianlong, Conrad, Fraser, Wen, Wei-Hua, Farr-Jones, Shauna, Smith, Theresa J, Brown, Jennifer L, Skerry, Janet C, Smith, Leonard A, Marks, James D
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.09.2021; MDPI
• Subjects: Adverse events; Affinity; Amino acids; Animals; Antibodies, Monoclonal - therapeutic use; antibody engineering; Antiinfectives and antibacterials; Antimicrobial agents; Antitoxins; botulinum neurotoxin; Botulinum toxin; Botulinum Toxins - immunology; Botulism; Botulism - immunology; Cattle; Chemotherapy; Epitopes; Female; Food contamination; Humans; Libraries; Mice; Molecular evolution; Monoclonal antibodies; Mosaics; Neurotoxins; Neutralization; oligoclonal antibodies; recombinant antibodies; Serogroup; serotype C botulism; serotype D botulism; Serotypes; Toxins; Yeast; Yeasts; antibody engineering; serotype D botulism; serotype C botulism; mouse neutralization assay; oligoclonal antibodies; recombinant antibodies; botulinum antitoxin; botulinum neurotoxin

Human botulism can be caused by botulinum neurotoxin (BoNT) serotypes A to G. Here, we present an antibody-based antitoxin composed of four human monoclonal antibodies (mAbs) against BoNT/C, BoNT/D, and their mosaic toxins. This work built on our success in generating protective mAbs to BoNT /A, B a...