Bioactive Compounds and Antioxidant Capacity in Anthocyanin-Rich Carrots: A Comparison between the Black Carrot and the Apulian Landrace "Polignano" Carrot

• Published in: Plants (Basel) Vol. 10; no. 3; p. 564
• Main Authors: Blando, Federica, Marchello, Stefano, Maiorano, Gabriele, Durante, Miriana, Signore, Angelo, Laus, Maura N, Soccio, Mario, Mita, Giovanni
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.03.2021; MDPI
• Subjects: acylated anthocyanin; Anthocyanins; antioxidant activity; Antioxidants; Bioactive compounds; Biodiversity; Biological activity; Carotene; Carotenoids; Carrots; Chlorogenic acid; Chromatography; Daucus; Food industry; Food processing industry; Fruits; Functional foods & nutraceuticals; Genotype & phenotype; Genotypes; Lutein; Malic acid; organic acid; Phenolic acids; Phenols; Phytochemicals; polyphenolic compounds; purple carrot; Reagents; Vegetables; Wildlife conservation; β-Carotene; Italy; Europe; glucose; purple carrot; carotenoids; vegetable biodiversity; polyphenolic compounds; acylated anthocyanin; fructose; organic acid; antioxidant activity
• DOI: 10.3390/plants10030564
• ISSN: 2223-7747

The carrot is one of the most cultivated vegetables in the world. Black or purple carrots contain acylated anthocyanins which are of special interest to the food industry for their stability and nutraceutical characteristics. Anthocyanin-rich fruits and vegetables have gained popularity in the last ten years, due to the health benefits they provide. In this paper, the characterizations of the bioactive compounds and antioxidant capacities of different anthocyanin-containing carrots (a black carrot-BC, and a local purple carrot, the "Polignano" carrot-PC), compared to the commercial orange carrot (OC) (lacking of anthocyanins), are reported. The anthocyanin profiles of the polyphenolic extracts of BC and PC were similar, but differences were observed at quantitative levels. The total anthocyanin content in BC was more than twice that in PC (13.84 ± 0.61 vs. 5.64 ± 0.48 mg K Eq. g DW). Phenolic acids (mostly chlorogenic acid) were also present at high level in anthocyanin-rich carrots compared to OC. High polyphenol content accounted also for a high reducing capacity (evaluated by Folin-Ciocalteu reagent, FCR), and antioxidant capacity (evaluated by TEAC and ORAC assays) which were the highest for BC (FCR value: 16.6 ± 1.1 mg GAE. g DW; TEAC: 76.6 ± 10.6 µmol TE. g DW; ORAC: 159.9 ± 3.3 µmol TE. g DW). All carrot genotypes (mostly OC) were rich in carotenoids (BC 0.14 ± 0.024; PC 0.33 ± 0.038; OC 1.29 ± 0.09 mg. g DW), with predominance of α and β-carotene, in OC, and lutein in BC. PC showed the highest malic acid and sugar (glucose plus fructose) content. In conclusion, while BC is remarkable for nutraceutical features, the local genotype ("Polignano" carrot) is worth considering in genetic biodiversity conservation programme.