In-site and Ex-site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation

• Published in: Plants (Basel) Vol. 10; no. 1; p. 137
• Main Authors: Chaâbene, Zayneb, Rorat, Agnieszka, Kriaa, Walid, Rekik, Imen, Mejdoub, Hafedh, Vandenbulcke, Franck, Elleuch, Amine
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12.01.2021; MDPI
• Subjects: Accumulation; Antioxidants; Arid regions; Arid zones; atmospheric contamination; Bioaccumulation; Biological magnification; Biomarkers; Cadmium; Chelating agents; Chromium; Controlled conditions; Copper; Enzymatic activity; Enzymes; Exposure; Flavonoids; Gene expression; Heavy metals; Homeostasis; integrative biomarkers; Metabolites; metal accumulation; Metal concentrations; metallic stress; Metals; Nitrogen; Oxidants; Oxidative stress; Oxidizing agents; Phoenix dactylifera; Phytotoxicity; Plantlets; Polyphenols; Proteins; Secondary metabolites; Signal transduction; Soil contamination; Soil improvement; Soil pollution; Translocation; Tunisia; atmospheric contamination; metallic stress; metal accumulation; integrative biomarkers; gene expression
• DOI: 10.3390/plants10010137
• ISSN: 2223-7747

As a tree of considerable importance in arid regions-date palm, L. survival in contaminated areas of Sfax city has drawn our attention. Leaf samples of the plants grown in the study area showed high levels of cadmium (Cd), copper (Cu), and chromium (Cr). On the basis of this finding, the cellular mechanisms that explain these metal accumulations were investigated in controlled conditions. After four months of exposure to Cd, Cr, or Cu, high bioconcentration and translocation factor (TF>1) have been shown for date palm plantlets exposed to Cd and low TF values were obtained for plantlets treated with Cr and Cu. Moreover, accumulation of oxidants and antioxidant enzyme activities occurred in exposed roots to Cu and Cd. Secondary metabolites, such as polyphenols and flavonoids, were enhanced in plants exposed at low metal concentrations and declined thereafter. Accumulation of flavonoids in cells may be correlated with the expression of the gene encoding Pdmate5, responsible for the transport of secondary metabolites, especially flavonoids. Other transporter genes responded positively to metal incorporation, especially but also and . The latter would be a new candidate gene sensitive to metallic stress in plants. Expressions of gene coding metal chelators were also investigated. and exhibited a strong induction in plants exposed to Cr. These modifications of the expression of some biochemical and molecular based-markers in date palm helped to better understand the ability of the plant to tolerate metals. They could be useful in assessing heavy metal contaminations in polluted soils and may improve accumulation capacity of other plants.