Mapping the DNA-Binding Motif of Scabin Toxin, a Guanine Modifying Enzyme from Streptomyces scabies

• Published in: Toxins Vol. 13; no. 1; p. 55
• Main Authors: Vatta, Maritza, Lyons, Bronwyn, Heney, Kayla A, Lidster, Taylor, Merrill, A Rod
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.01.2021; MDPI
• Subjects: Adenosine diphosphate; Apoptosis; Arginine; bacterial toxins; Binding; bioinformatics; Crystal structure; crystallography; Deoxyribonucleic acid; DNA; DNA modification; DNA-binding motif; enzyme kinetics; Enzymes; Fluorescence; Guanine; Lysine; Molecular dynamics; NAD; Parasitic diseases; Proteins; Reaction mechanisms; Residues; Ribonucleic acid; Ribose; RNA; Streptomyces scabies; Substrates; Toxins; Virulence; Virulence factors; crystallography; molecular modeling; bioinformatics; DNA modification; bacterial toxins; enzyme kinetics; DNA-binding motif

Scabin is a mono-ADP-ribosyltransferase toxin/enzyme and possible virulence factor produced by the agriculture pathogen, . Recently, molecular dynamic approaches and MD simulations revealed its interaction with both NAD and DNA substrates. An Essential Dynamics Analysis identified a crab-claw-like m...