A single prolonged stress paradigm produces enduring impairments in social bonding in monogamous prairie voles

• Published in: Behavioural brain research Vol. 315; pp. 83 - 93
• Main Authors: Arai, Aki, Hirota, Yu, Miyase, Naoki, Miyata, Shiori, Young, Larry J., Osako, Yoji, Yuri, Kazunari, Mitsui, Shinichi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.2016
• Subjects: Animals; Arvicolinae; Brain - metabolism; Brain - pathology; Disease Models, Animal; Exploratory Behavior - drug effects; Female; Male; Microtus; Microtus ochrogaster; Neurons - metabolism; Oxytocin - metabolism; Pair Bond; Paroxetine; Paroxetine - therapeutic use; Partner preference; PTSD; Receptors, Glucocorticoid - metabolism; Receptors, Vasopressin - metabolism; Serotonin; Serotonin Uptake Inhibitors - therapeutic use; Social behavior; SSRI; Stress Disorders, Post-Traumatic - physiopathology; Supraoptic Nucleus - pathology; Time Factors; Tyrosine 3-Monooxygenase - metabolism; PBS; Paroxetine; Serotonin; MR; LS; MeA; IR; PVN; GR; V1aR; PBS-T; PTSD; SSRI; Partner preference; SON; Social behavior; pBNST; TH; NAcc; SPS; VP
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2016.08.022

•Single prolonged stress (SPS) disturbed the formation of pair bond in prairie voles.•The administration of SSRI prevented the disturbance of pair bonding.•SPS transiently decreased oxytocin levels in the SON.•SPS affected the response of oxytocin neurons in the SON to exposure to a partner.•SPS-treated voles may be suitable to study the effect of trauma on social bonding. Traumatic events such as natural disasters, violent crimes, tragic accidents, and war, can have devastating impacts on social relationships, including marital partnerships. We developed a single prolonged stress (SPS) paradigm, which consisted of restraint, forced swimming, and ether anesthesia, to establish an animal model relevant to post-traumatic stress disorder. We applied a SPS paradigm to a monogamous rodent, the prairie vole (Microtus ochrogaster) in order to determine whether a traumatic event affects the establishment of pair bonds. We did not detect effects of the SPS treatment on anhedonic or anxiety-like behavior. Sham-treated male voles huddled with their partner females, following a 6day cohabitation, for a longer duration than with a novel female, indicative of a pair bond. In contrast, SPS-treated voles indiscriminately huddled with the novel and partner females. Interestingly, the impairment of pair bonding was rescued by oral administration of paroxetine, a selective serotonin reuptake inhibitor (SSRI), after the SPS treatment. Immunohistochemical analyses revealed that oxytocin immunoreactivity (IR) was significantly decreased in the supraoptic nucleus (SON), but not in the paraventricular nucleus (PVN), 7days after SPS treatment, and recovered 14days after SPS treatment. After the presentation of a partner female, oxytocin neurons labeled with Fos IR was significantly increased in SPS-treated voles compared with sham-treated voles regardless of paroxetine administration. Our results suggest that traumatic events disturb the formation of pair bond possibly through an interaction with the serotonergic system, and that SSRIs are candidates for the treatment of social problems caused by traumatic events. Further, a vole SPS model may be useful for understanding mechanisms underlying the impairment of social bonding by traumatic events.