Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles

• Published in: Journal of immunological methods Vol. 443; pp. 33 - 44
• Main Authors: Brown, Eric P., Dowell, Karen G., Boesch, Austin W., Normandin, Erica, Mahan, Alison E., Chu, Thach, Barouch, Dan H., Bailey-Kellogg, Chris, Alter, Galit, Ackerman, Margaret E.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2017; Elsevier
• Subjects: ADCC; agglutination; AIDS Vaccines - administration & dosage; AIDS Vaccines - immunology; Animals; Antibody; Antibody Affinity; Antibody-Dependent Cell Cytotoxicity; antigens; Binding Sites, Antibody; Case-Control Studies; clinical trials; disease course; Disease Models, Animal; Effector function; enzyme-linked immunosorbent assay; Flow Cytometry; High-Throughput Screening Assays - methods; HIV - immunology; HIV Antibodies - blood; HIV Antibodies - immunology; HIV Infections - blood; HIV Infections - immunology; HIV Infections - therapy; Human immunodeficiency virus; Humans; humoral immunity; IgG; immune system; Immunity, Humoral - drug effects; Immunization; Immunoglobulin Fc Fragments - blood; Immunoglobulin Fc Fragments - immunology; Immunologic Techniques; Macaca mulatta; monoclonal antibodies; neutralization; patients; Phagocytosis; Protein Binding; Receptors, IgG - genetics; Receptors, IgG - immunology; Receptors, IgG - metabolism; Research Paper; Simian Acquired Immunodeficiency Syndrome - blood; Simian Acquired Immunodeficiency Syndrome - immunology; Simian Acquired Immunodeficiency Syndrome - therapy; Simian Immunodeficiency Virus - immunology; therapeutics; Vaccines; IgG; Vaccines; Antibody; Effector function; ADCC; Phagocytosis
• ISSN: 0022-1759; 1872-7905; 1872-7905
• DOI: 10.1016/j.jim.2017.01.010

Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody response is of critical importance in attempting to understand and predict the efficacy of novel vaccine candidates. Historically, antibody respo...