Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells

• Published in: Nature methods Vol. 11; no. 3; pp. 319 - 324
• Main Authors: Kulak, Nils A, Pichler, Garwin, Paron, Igor, Nagaraj, Nagarjuna, Mann, Matthias
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2014; Nature Publishing Group
• Subjects: 38/56; 631/1647/2067; 631/337/475; 631/45/475; 82/16; 82/58; 82/81; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical Engineering/Biotechnology; Cells; Contamination; DNA Contamination; Eukaryotes; Eukaryotic Cells - metabolism; Gene Dosage - genetics; HeLa Cells; Humans; Life Sciences; Mass spectrometry; Numbers; Peptides; Physiological aspects; Proteomics; Proteomics - methods; Reproducibility of Results; Saccharomyces cerevisiae Proteins - genetics; Yeasts; Germany

A streamlined, robust sample-preparation method for mass spectrometry–based proteome analysis is reported. All sample preparation steps are carried out in a single enclosed reactor, reducing the potential for contamination and losses, and enabling comprehensive proteome coverage. Mass spectrometry (...