Human miR‐1228 as a stable endogenous control for the quantification of circulating microRNAs in cancer patients

• Published in: International journal of cancer Vol. 135; no. 5; pp. 1187 - 1194
• Main Authors: Hu, Jie, Wang, Zheng, Liao, Bo‐Yi, Yu, Lei, Gao, Xue, Lu, Shaohua, Wang, Shuyang, Dai, Zhi, Zhang, Xin, Chen, Qing, Qiu, Shuang‐Jian, Wu, Ying, Zhu, Hongguang, Fan, Jia, Zhou, Jian, Wang, Jiping
• Format: Journal Article
• Language: English
• Published: Hoboken, NJ Wiley-Blackwell 01.09.2014; Wiley Subscription Services, Inc
• Subjects: Adult; Biological and medical sciences; Biomarkers; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Breast cancer; Cancer; circulating microRNA; Cohort Studies; Control Groups; endogenous control; Female; Gene Expression Profiling; Genes, Essential; Hematology; Hepatitis B, Chronic - blood; Hepatitis B, Chronic - genetics; Humans; Male; Medical research; Medical sciences; MicroRNAs; MicroRNAs - blood; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Neoplasms - blood; normalization; Oligonucleotide Array Sequence Analysis; qRT‐PCR; Real-Time Polymerase Chain Reaction; Signal Transduction; Tumors; Human; RNA interference; Micro RNA; qRT-PCR; Quantization; Malignant tumor; endogenous control; Gene silencing; Cancerology; Endogenous; normalization; circulating microRNA; Cancer; Real time polymerase chain reaction; cancer

Circulating microRNAs are promising biomarkers for non‐invasive testing and dynamic monitoring in cancer patients. However, no consensus exists regarding the normalization of circulating microRNAs in the quantification, making the results incomparable. We investigated global circulating microRNA pro...