Prolonged induction of c-fos in neuropeptide Y-and somatostatin-immunoreactive neurons of the rat dentate gyrus after electroconvulsive stimulation

• Published in: Brain research Vol. 720; no. 1; pp. 111 - 119
• Main Authors: Woldbye, David P.D., Greisen, Mia H., Bolwig, Tom G., Larsen, Philip J., Mikkelsen, Jens D.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 13.05.1996; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; c-fos; Dentate gyrus; Dentate Gyrus - cytology; Dentate Gyrus - metabolism; Dentate Gyrus - physiology; Diseases of the nervous system; Electric Stimulation; Electroconvulsive seizure; Electroconvulsive stimulation; Immunohistochemistry; In Situ Hybridization; Male; Medical sciences; Neurons - metabolism; Neurons - physiology; Neuropeptide Y; Neuropeptide Y - metabolism; Oligonucleotide Probes; Proto-Oncogene Proteins c-fos - biosynthesis; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Wistar; RNA, Messenger - biosynthesis; Seizures - metabolism; Somatostatin; Somatostatin - metabolism; Neuropeptide Y; Electroconvulsive stimulation; c-fos; Dentate gyrus; Somatostatin; Electroconvulsive seizure; Nervous system diseases; Electroconvulsive therapy; Rat; Epilepsy; Rodentia; Central nervous system; Depression; Cerebral disorder; Vertebrata; Mammalia; Animal; Central nervous system disease; Hippocampus; Protooncogene; Brain (vertebrata); Immediate early gene
• DOI: 10.1016/0006-8993(96)00158-8
• ISSN: 0006-8993

Induction of c-fos mRNA and Fos was studied in the hilus and granular layer of the dentate gyrus at various times up to 24 h after single electroconvulsive stimulation (ECS) using in situ hybridization and immunocytochemistry. In both areas of the dentate gyrus, a prominent induction of c-fos mRNA and Fos was observed. Compared to the granular layer, however, c-fos mRNA and Fos in hilar cells reached maximum later and remained elevated considerably longer. Several neurochemically distinct populations of hilar neurons have been described, some of which contain neuropeptide Y (NPY) and/or somatostatin (SS). Using double-labelling immunocytochemistry, we examined to what extent Fos was induced in these hilar neurons after ECS. Although a minor population of non-NPY non-SS cells displayed Fos induction early after ECS, prolonged induction of Fos almost exclusively occurred in NPY or SS neurons. The Fos-immunoreactive NPY or SS neurons only amounted to about 50% of the total hilar population of NPY or SS neurons. The present observations suggest that a subpopulation of hilar NPY and SS neurons may be central to the actions of electroconvulsive seizures in the dentate gyrus.