An Infectious cDNA Clone of SARS-CoV-2

• Published in: Cell host & microbe Vol. 27; no. 5; pp. 841 - 848.e3
• Main Authors: Xie, Xuping, Muruato, Antonio, Lokugamage, Kumari G., Narayanan, Krishna, Zhang, Xianwen, Zou, Jing, Liu, Jianying, Schindewolf, Craig, Bopp, Nathen E., Aguilar, Patricia V., Plante, Kenneth S., Weaver, Scott C., Makino, Shinji, LeDuc, James W., Menachery, Vineet D., Shi, Pei-Yong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.05.2020
• Subjects: Animals; antiviral; Antiviral Agents - therapeutic use; Betacoronavirus - genetics; Betacoronavirus - pathogenicity; Chlorocebus aethiops; Clone Cells; coronavirus; Coronavirus Infections - drug therapy; Coronavirus Infections - virology; COVID-19; DNA, Complementary - genetics; Genes, Reporter - genetics; Genome, Viral - genetics; Interferons - therapeutic use; Organisms, Genetically Modified - genetics; Organisms, Genetically Modified - pathogenicity; Pandemics; Pneumonia, Viral - drug therapy; Pneumonia, Viral - virology; RNA, Viral - genetics; SARS-CoV; SARS-CoV-2; vaccine; Vero Cells - virology; Virus Replication - physiology; COVID-19; coronavirus; SARS-CoV-2; vaccine; SARS-CoV; antiviral

The ongoing pandemic of COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), underscores the urgency to develop experimental systems for studying this virus and identifying countermeasures. We report a reverse genetic system for SARS-CoV-2. Seven complimentary DNA (cDNA)...