Saccharomyces cerevisiae Signature Genes for Predicting Nitrogen Deficiency during Alcoholic Fermentation

• Published in: Applied and Environmental Microbiology Vol. 73; no. 16; pp. 5363 - 5369
• Main Authors: Mendes-Ferreira, A, del Olmo, M, García-Martínez, J, Jiménez-Martí, E, Leão, C, Mendes-Faia, A, Pérez-Ortín, J.E
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2007
• Subjects: Alcohols - metabolism; Biological and medical sciences; Fermentation; Fermentation - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Fungal; Genes, Fungal; Genomics; Microbiology; Models, Genetic; Nitrogen; Nitrogen - deficiency; Nitrogen - metabolism; Oligonucleotide Array Sequence Analysis; Physiology and Biotechnology; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Vitaceae; Yeast; Fungi; Yeast; Alcoholic fermentation; Gene; Deficiency; Ascomycetes; Nitrogen; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01029-07

Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.