Chelation and determination of labile iron in primary hepatocytes by pyridinone fluorescent probes

• Published in: Biochemical journal Vol. 395; no. 1; pp. 49 - 55
• Main Authors: Ma, Yongmin, de Groot, Herbert, Liu, Zudong, Hider, Robert C, Petrat, Frank
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 01.04.2006
• Subjects: Animals; Calibration; Cell-Free System; Cells, Cultured; Fluorescent Dyes - analysis; Fluorescent Dyes - chemistry; Hepatocytes - cytology; Hepatocytes - metabolism; Iron - analysis; Iron - chemistry; Iron - metabolism; Iron Chelating Agents - analysis; Iron Chelating Agents - chemistry; Male; Pyridones - analysis; Pyridones - chemistry; Pyridones - metabolism; Rats; Rats, Wistar

A series of fluorescent iron chelators has been synthesized such that a fluorescent function is covalently linked to a 3-hydroxypyridin-4-one. In the present study, the fluorescent iron chelators were loaded into isolated rat hepatocytes. The intracellular fluorescence was not only quenched by an ad...