Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping

• Published in: Journal of clinical microbiology Vol. 53; no. 8; pp. 2427 - 2432
• Main Authors: Iguchi, Atsushi, Iyoda, Sunao, Seto, Kazuko, Morita-Ishihara, Tomoko, Scheutz, Flemming, Ohnishi, Makoto
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.2015
• Subjects: Animals; Bacteriology; DNA Primers - genetics; Escherichia coli; Escherichia coli - classification; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Genotyping Techniques - methods; Humans; Multiplex Polymerase Chain Reaction - methods; O Antigens - genetics; Sensitivity and Specificity; Serogroup; Serotyping - methods

The O serogrouping of pathogenic Escherichia coli is a standard method for subtyping strains for epidemiological studies and enhancing phylogenetic studies. In particular, the identification of strains of the same O serogroup is essential in outbreak investigations and surveillance. In a previous st...