Whole-transcriptome analysis of endothelial to hematopoietic stem cell transition reveals a requirement for Gpr56 in HSC generation

• Published in: The Journal of experimental medicine Vol. 212; no. 1; pp. 93 - 106
• Main Authors: Solaimani Kartalaei, Parham, Yamada-Inagawa, Tomoko, Vink, Chris S, de Pater, Emma, van der Linden, Reinier, Marks-Bluth, Jonathon, van der Sloot, Anthon, van den Hout, Mirjam, Yokomizo, Tomomasa, van Schaick-Solernó, M Lucila, Delwel, Ruud, Pimanda, John E, van IJcken, Wilfred F J, Dzierzak, Elaine
• Format: Journal Article
• Language: English
• Published: United States The Rockefeller University Press 12.01.2015
• Subjects: Animals; Cell Line; Cell Transdifferentiation - genetics; Cells, Cultured; Cercopithecus aethiops; CHO Cells; COS Cells; Cricetinae; Cricetulus; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Endothelial Cells - cytology; Endothelial Cells - metabolism; Female; Gene Expression Profiling - methods; Gene Ontology; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Humans; In Situ Hybridization; Mice, Inbred C57BL; Microscopy, Confocal; Oligonucleotide Array Sequence Analysis; Receptors, G-Protein-Coupled - genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, RNA - methods; Up-Regulation

Hematopoietic stem cells (HSCs) are generated via a natural transdifferentiation process known as endothelial to hematopoietic cell transition (EHT). Because of small numbers of embryonal arterial cells undergoing EHT and the paucity of markers to enrich for hemogenic endothelial cells (ECs [HECs]),...