Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension

• Published in: Biochemical and biophysical research communications Vol. 451; no. 1; pp. 8 - 14
• Main Authors: Shao, Dongmin, Perros, Frédéric, Caramori, Gaetano, Meng, Chao, Dormuller, Peter, Chou, Pai-Chien, Church, Colin, Papi, Alberto, Casolari, Paolo, Welsh, David, Peacock, Andrew, Humbert, Marc, Adcock, Ian M., Wort, Stephen J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.08.2014
• Subjects: 60 APPLIED LIFE SCIENCES; Base Sequence; Binding Sites; blood serum; Case-Control Studies; CELL MEMBRANES; Cells, Cultured; CHROMATIN; COMPLEXES; DEATH; endothelial cells; Endothelial Cells - metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Familial Primary Pulmonary Hypertension - metabolism; Familial Primary Pulmonary Hypertension - pathology; gene expression; Gene Expression Regulation; GENES; histones; homeodomain proteins; Human endothelial cells; HUMAN POPULATIONS; Humans; HYPERTENSION; IL-33; immunohistochemistry; INFLAMMATION; Inflammation Mediators - metabolism; Interleukin-1 Receptor-Like 1 Protein; Interleukin-33; interleukin-6; Interleukin-6 - genetics; Interleukin-6 - metabolism; Interleukins - blood; Interleukins - genetics; Interleukins - metabolism; Lung - metabolism; LUNGS; MESSENGER-RNA; METHYL TRANSFERASES; methyltransferases; Molecular Sequence Data; Nuclear repressor; PATHOGENESIS; PATIENTS; POLYCYCLIC AROMATIC HYDROCARBONS; precipitin tests; Promoter Regions, Genetic; Pulmonary hypertension; RECEPTORS; Receptors, Cell Surface - blood; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; repressor proteins; Signal Transduction; small interfering RNA; Soluble ST2; Human endothelial cells; Soluble ST2; Nuclear repressor; IL-33; Pulmonary hypertension

•Nuclear IL-33 expression is reduced in vascular endothelial cells from PAH patients.•Knockdown of IL-33 leads to increased IL-6 and sST2 mRNA expression.•IL-33 binds homeobox motifs in target gene promoters and recruits repressor proteins. Idiopathic pulmonary arterial hypertension (IPAH) is an inc...