Research progress on siRNA delivery with nonviral carriers

• Published in: International journal of nanomedicine Vol. 6; no. default; pp. 1017 - 1025
• Main Authors: Gao, Yan, Liu, Xin-Ling, Li, Xiao-Rong
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Press 01.01.2011; Dove Medical Press
• Subjects: Animals; biocompatibility; delivery system; Drug Carriers - administration & dosage; Drug Delivery Systems; gene therapy; Genetic Therapy - methods; Humans; nanoparticles; nonviral vector; Review; RNA, Small Interfering - administration & dosage; small interfering RNA; gene therapy; delivery system; small interfering RNA; nanoparticles; biocompatibility; nonviral vector
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/ijn.s17040

RNA interference is a powerful method for the knockdown of pathologically relevant genes. Small interfering RNAs (siRNAs) have been widely demonstrated as effective biomedical genetic-therapy applications for many diseases. Unfortunately, siRNA duplexes are not ideal drug-like molecules. Problems hindering their effective application fundamentally lie in their delivery, stability, and off-target effects. Delivery systems provide solutions to many of the challenges facing siRNA therapeutics. Due to some fatal disadvantages of viral vectors, nonviral carriers have been studied extensively. Aside from liposomes, nanoparticles and cationic polymer carriers have exhibited improved in vivo stability, better biocompatibility, and efficiency for gene silencing with less cellular toxicity. They may represent a promising strategy for siRNA-based therapies, especially as nanomaterials. The present review also summarizes other methods of siRNA delivery and the side effects of the nanoparticles.