Stimulation of Bone Resorption in vitro by Synthetic Transforming Growth Factor-Alpha

• Published in: Science (American Association for the Advancement of Science) Vol. 228; no. 4702; pp. 1007 - 1009
• Main Authors: Ibbotson, K. J., Twardzik, D. R., D'Souza, S. M., Hargreaves, W. R., Todaro, G. J., Mundy, G. R.
• Format: Journal Article
• Language: English
• Published: Washington, DC The American Association for the Advancement of Science 24.05.1985; American Association for the Advancement of Science
• Subjects: Animals; Biological and medical sciences; Bone and Bones - drug effects; Bone diseases; Bone resorption; Bone Resorption - drug effects; Bones; Cancer; Cancer research; Cell physiology; Cell research; Cytological research; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Gels; History of medicine; History, 20th Century; Hypercalcemia; Kinetics; Low molecular weights; Molecular and cellular biology; Molecular Weight; Oncology, Experimental; Organ Culture Techniques; Peptides - chemical synthesis; Peptides - pharmacology; Rats; Receptors; Responses to growth factors, tumor promotors, other factors; Transforming Growth Factors; Tumors; Ungulates; Calcium; Tissue culture; Resorption; Tumor; Bone; Biological activity; Release; Growth factor
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.3859011

Experiments were conducted to test the hypothesis that tumor-derived transforming growth factor-alpha (TGF-α) is responsible for the increased bone resorption and hypercalcemia seen in some malignant diseases. Homogeneous synthetic TGF-α prepared by the solid-phase synthesis method stimulated bone resorption directly in vitro in a concentration-dependent manner. Incubation times of 72 hours or more were required to stimulate resorption, which is similar to the time course of bone resorption by epidermal growth factor.