Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody Recognition

• Published in: Cell host & microbe Vol. 29; no. 1; pp. 44 - 57.e9
• Main Authors: Greaney, Allison J., Starr, Tyler N., Gilchuk, Pavlo, Zost, Seth J., Binshtein, Elad, Loes, Andrea N., Hilton, Sarah K., Huddleston, John, Eguia, Rachel, Crawford, Katharine H.D., Dingens, Adam S., Nargi, Rachel S., Sutton, Rachel E., Suryadevara, Naveenchandra, Rothlauf, Paul W., Liu, Zhuoming, Whelan, Sean P.J., Carnahan, Robert H., Crowe, James E., Bloom, Jesse D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.01.2021; Cell Press
• Subjects: Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - metabolism; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; antibody escape; antigenic evolution; Binding Sites; deep mutational scanning; Epitopes - immunology; Gene Library; High-Throughput Nucleotide Sequencing; Humans; Protein Domains; Saccharomyces cerevisiae - genetics; SARS-CoV-2; SARS-CoV-2 - genetics; SARS-CoV-2 - immunology; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; Spike Glycoprotein, Coronavirus - metabolism; antibody escape; SARS-CoV-2; deep mutational scanning; antigenic evolution

Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are being developed as therapeutics and are a major contributor to neutralizing antibody responses elicited by infection. Here, we describe a deep mutational scanning method to map how all amino-acid mutations in the RBD affect...