Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk

• Published in: Journal of dairy science Vol. 101; no. 7; pp. 5770 - 5779
• Main Authors: Han, Jiaojiao, Zhang, Lei, Hu, Liming, Xing, Keyu, Lu, Xuefei, Huang, Youju, Zhang, Jiawei, Lai, Weihua, Chen, Tao
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2018
• Subjects: 3,3′,5,5′-tetramethylbenzidine; Animals; catalytic activity; chemical species; cost effectiveness; Escherichia coli O157; Escherichia coli O157 - isolation & purification; Escherichia coli O157:H7; Food Analysis - methods; Food Microbiology; lateral flow assay; milk; Milk - microbiology; nanoparticles; nanozyme; palladium; pathogens; platinum; nanozyme; 3,3′,5,5′-tetramethylbenzidine; lateral flow assay; Escherichia coli O157:H7

Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by u...