Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk
Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by u...
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| Published in | Journal of dairy science Vol. 101; no. 7; pp. 5770 - 5779 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
01.07.2018
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 102 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples. |
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| AbstractList | Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 102 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples. Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 102 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples.Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 102 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples. Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 10 cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples. Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA was improved by applying the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 × 10² cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples. |
| Author | Huang, Youju Lu, Xuefei Han, Jiaojiao Hu, Liming Zhang, Jiawei Chen, Tao Lai, Weihua Xing, Keyu Zhang, Lei |
| Author_xml | – sequence: 1 givenname: Jiaojiao surname: Han fullname: Han, Jiaojiao organization: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China – sequence: 2 givenname: Lei surname: Zhang fullname: Zhang, Lei organization: Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China – sequence: 3 givenname: Liming surname: Hu fullname: Hu, Liming organization: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China – sequence: 4 givenname: Keyu surname: Xing fullname: Xing, Keyu organization: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China – sequence: 5 givenname: Xuefei surname: Lu fullname: Lu, Xuefei organization: Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China – sequence: 6 givenname: Youju surname: Huang fullname: Huang, Youju email: yjhuang@nimte.ac.cn organization: Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China – sequence: 7 givenname: Jiawei surname: Zhang fullname: Zhang, Jiawei organization: Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China – sequence: 8 givenname: Weihua surname: Lai fullname: Lai, Weihua email: talktolaiwh@163.com organization: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China – sequence: 9 givenname: Tao surname: Chen fullname: Chen, Tao email: tao.chen@nimte.ac.cn organization: Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China |
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| Copyright | 2018 American Dairy Science Association Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
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| Keywords | nanozyme 3,3′,5,5′-tetramethylbenzidine lateral flow assay Escherichia coli O157:H7 |
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| SubjectTerms | 3,3′,5,5′-tetramethylbenzidine Animals catalytic activity chemical species cost effectiveness Escherichia coli O157 Escherichia coli O157 - isolation & purification Escherichia coli O157:H7 Food Analysis - methods Food Microbiology lateral flow assay milk Milk - microbiology nanoparticles nanozyme palladium pathogens platinum |
| Title | Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk |
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