Spectra of superoxide-induced mutations in the lacI gene of a wild-type and a mutM strain of Escherichia coli K-12

• Published in: Mutation research Vol. 326; no. 2; p. 175
• Main Authors: Ono, T, Negishi, K, Hayatsu, H
• Format: Journal Article
• Language: English
• Published: Netherlands 01.02.1995
• Subjects: Base Sequence; beta-Galactosidase - genetics; DNA Mutational Analysis; DNA Primers; DNA Repair; DNA-Formamidopyrimidine Glycosylase; Dose-Response Relationship, Drug; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli Proteins; Guanine - analogs & derivatives; Guanine - biosynthesis; Lac Operon - drug effects; Lac Operon - genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutagens - toxicity; N-Glycosyl Hydrolases - metabolism; Point Mutation; Superoxides - toxicity; Vitamin K - toxicity

We have analyzed the spectra of superoxide-induced mutations in the chromosomal lacI gene of a wild-type and a mutM strain of Escherichia coli K-12. The mutM strain is known to be deficient in removing 8-hydroxyguanine from DNA. An intracellular superoxide-generating agent, menadione, was used to ca...