Rapid Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates Directly from Clinical Samples

• Published in: Journal of clinical microbiology Vol. 53; no. 7; pp. 2230 - 2237
• Main Authors: Brown, Amanda C., Bryant, Josephine M., Einer-Jensen, Katja, Holdstock, Jolyon, Houniet, Darren T., Chan, Jacqueline Z. M., Depledge, Daniel P., Nikolayevskyy, Vladyslav, Broda, Agnieszka, Stone, Madeline J., Christiansen, Mette T., Williams, Rachel, McAndrew, Michael B., Tutill, Helena, Brown, Julianne, Melzer, Mark, Rosmarin, Caryn, McHugh, Timothy D., Shorten, Robert J., Drobniewski, Francis, Speight, Graham, Breuer, Judith
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.07.2015
• Subjects: Bacteriological Techniques - methods; Drug Resistance, Bacterial; Genotyping Techniques - methods; Humans; Lithuania; Mycobacteriology and Aerobic Actinomycetes; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Sequence Analysis, DNA - methods; Specimen Handling - methods; Sputum - microbiology; Time Factors; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - microbiology; United Kingdom

The rapid identification of antimicrobial resistance is essential for effective treatment of highly resistant Mycobacterium tuberculosis . Whole-genome sequencing provides comprehensive data on resistance mutations and strain typing for monitoring transmission, but unlike for conventional molecular...