Cerebrospinal Fluid and Peripheral Blood Lymphomonocyte Single-Cell Transcriptomics in a Subject with Multiple Sclerosis Acutely Infected with HIV

• Published in: International journal of molecular sciences Vol. 25; no. 19; p. 10459
• Main Authors: Pinnetti, Carmela, Rozera, Gabriella, Messina, Francesco, Spezia, Pietro Giorgio, Lazzari, Elisabetta, Fabeni, Lavinia, Chillemi, Giovanni, Pietrucci, Daniele, Haggiag, Shalom, Mastrorosa, Ilaria, Vergori, Alessandra, Girardi, Enrico, Antinori, Andrea, Maggi, Fabrizio, Abbate, Isabella
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.10.2024; MDPI
• Subjects: Adult; Brief Report; Cells; Cerebrospinal fluid; Chemokines; Cluster analysis; Comorbidity; Development and progression; Disease; Drug therapy; Gene expression; Gene Expression Profiling - methods; HIV; HIV infection; HIV Infections - cerebrospinal fluid; HIV Infections - genetics; HIV Infections - virology; HIV-1 - genetics; Human immunodeficiency virus; Humans; Immune response; Infections; Kinases; Leukocytes; Leukocytes, Mononuclear - metabolism; Leukocytes, Mononuclear - virology; Male; Multiple sclerosis; Multiple Sclerosis - blood; Multiple Sclerosis - cerebrospinal fluid; Multiple Sclerosis - genetics; Multiple Sclerosis - virology; Neurodegeneration; Patients; Plasma; Proteins; Single-Cell Analysis - methods; Transcriptome; Italy; immune dysregulation in infectious diseases; interactions between infective agents and immune responses

Signatures of neurodegeneration in clinical samples from a subject with multiple sclerosis (MS) acutely infected with HIV were investigated with single-cell transcriptomics using 10X Chromium technology. Sequencing was carried out on NovaSeq-TM, and the analysis was performed with Cell Ranger softwa...