Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants

• Published in: Journal of virology Vol. 88; no. 21; pp. 12364 - 12373
• Main Authors: Matsuzaki, Yoko, Sugawara, Kanetsu, Nakauchi, Mina, Takahashi, Yoshimasa, Onodera, Taishi, Tsunetsugu-Yokota, Yasuko, Matsumura, Takayuki, Ato, Manabu, Kobayashi, Kazuo, Shimotai, Yoshitaka, Mizuta, Katsumi, Hongo, Seiji, Tashiro, Masato, Nobusawa, Eri
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.11.2014
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Epitope Mapping - methods; Hemagglutination Inhibition Tests; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - immunology; Influenza virus; Mice, Inbred BALB C; Molecular Sequence Data; Mutant Proteins - genetics; Mutant Proteins - immunology; Pathogenesis and Immunity; RNA, Viral - genetics; Selection, Genetic; Sequence Analysis, DNA; Virus Cultivation; ASW, Caribbean Sea, Greater Antilles, Puerto Rico

We determined the antigenic structure of pandemic influenza A(H1N1)pdm09 virus hemagglutinin (HA) using 599 escape mutants that were selected using 16 anti-HA monoclonal antibodies (MAbs) against A/Narita/1/2009. The sequencing of mutant HA genes revealed 43 amino acid substitutions at 24 positions...