Bone sialoprotein (BSP) secretion and osteoblast differentiation: relationship to bromodeoxyuridine incorporation, alkaline phosphatase, and matrix deposition

• Published in: The journal of histochemistry and cytochemistry Vol. 41; no. 2; pp. 183 - 191
• Main Authors: Bianco, P, Riminucci, M, Bonucci, E, Termine, JD, Robey, PG
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.02.1993; SAGE Publications; Histochemical Society
• Subjects: Alkaline Phosphatase - metabolism; Animals; Biological and medical sciences; Bone Matrix - metabolism; Bromodeoxyuridine - metabolism; Cell Differentiation; Cell Division; Embryology: invertebrates and vertebrates. Teratology; Fundamental and applied biological sciences. Psychology; Histocytochemistry; Immunoenzyme Techniques; Integrin-Binding Sialoprotein; Organogenesis. Fetal development; Organogenesis. Physiological fonctions; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis; Rats; Rats, Wistar; Sialoglycoproteins - secretion; Space life sciences; Alkaline phosphatase; Embryonic development; Rat; Enzyme; Rodentia; Gene expression; Vertebrata; Mammalia; Sialoproteins; Osteoblast; Fetus; Skeleton; Bone; Differentiation; Osteogenesis

We defined two distinct maturational compartments (proliferative and secretory) of osteogenic cells in vivo on the basis of ALP activity, BrdU incorporation, cell shape, and BSP production. BSP immunoreactivity was found to mark cells in the secretory but not in the proliferative compartment. We est...