The Establishment of an Assay to Measure DNA Polymerase-Catalyzed Repair of UVB-Induced DNA Damage in Skin Cells and Screening of DNA Polymerase Enhancers from Medicinal Plants

• Published in: International journal of molecular sciences Vol. 17; no. 5; p. 667
• Main Authors: Ikeoka, Sawako, Nakahara, Tatsuo, Iwahashi, Hiroyasu, Mizushina, Yoshiyuki
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 04.05.2016
• Subjects: Cell Line; DNA Damage; DNA Repair - drug effects; DNA-Directed DNA Polymerase - metabolism; Humans; Keratinocytes - drug effects; Keratinocytes - metabolism; Keratinocytes - radiation effects; Plant Extracts - pharmacology; Plants, Medicinal - chemistry; Ultraviolet Rays - adverse effects; enzyme enhancer; DNA polymerases; normal human epidermal keratinocytes (NHEK); rose myrtle; piceatannol; ultraviolet B (UVB)

An in vitro assay method was established to measure the activity of cellular DNA polymerases (Pols) in cultured normal human epidermal keratinocytes (NHEKs) by modifying Pol inhibitor activity. Ultraviolet (UV) irradiation enhanced the activity of Pols, especially DNA repair-related Pols, in the cel...