The development of a new family of intracellular calcium probes

• Published in: Cell calcium (Edinburgh) Vol. 15; no. 2; pp. 190 - 198
• Main Authors: Iatridou, H., Foukaraki, E., Kuhn, M.A., Marcus, E.M., Haugland, R.P., Katerinopoulos, H.E.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier India Pvt Ltd 01.02.1994; Elsevier
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Calcium - analysis; Coumarins; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Fundamental and applied biological sciences. Psychology; Mice; Analysis method; Calcium; Probe characterization; Spectral properties; Intracellular; Chemical synthesis; Quantitative analysis

Four new potential fluorescent probes for Ca 2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding affinities for Ca 2+ determined. The most promising derivative for intracellular use, BTC, exhibits an excitation wavelength shift from 462 nm to...