The development of a new family of intracellular calcium probes
Four new potential fluorescent probes for Ca 2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding affinities for Ca 2+ determined. The most promising derivative for intracellular use, BTC, exhibits an excitation wavelength shift from 462 nm to...
Saved in:
| Published in | Cell calcium (Edinburgh) Vol. 15; no. 2; pp. 190 - 198 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford
Elsevier India Pvt Ltd
01.02.1994
Elsevier |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Four new potential fluorescent probes for Ca
2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding affinities for Ca
2+ determined. The most promising derivative for intracellular use, BTC, exhibits an excitation wavelength shift from 462 nm to 401 nm on binding Ca
2+, with an emission wavelength of 530 nm. The quantum yield of this probe increases from 0.07 as the free indicator to 0.12 on binding Ca
2+. BTC, loaded as its tetraacetoxymethyl ester (AM ester) into mouse myeloma P3X cells, responded only when cytoplasmic Ca
2+ exceeded typical intracellular calcium concentrations. The dye, therefore, appears to be useful in excitatory cells or extracellular spaces with intracellular calcium concentrations high enough to saturate typical excitation ratio Ca
2+ indicators such as Fura-2. |
|---|---|
| AbstractList | Four new potential fluorescent probes for Ca2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding affinities for Ca2+ determined. The most promising derivative for intracellular use, BTC, exhibits an excitation wavelength shift from 462 nm to 401 nm on binding Ca2+, with an emission wavelength of 530 nm. The quantum yield of this probe increases from 0.07 as the free indicator to 0.12 on binding Ca2+. BTC, loaded as its tetraacetoxymethyl ester (AM ester) into mouse myeloma P3X cells, responded only when cytoplasmic Ca2+ exceeded typical intracellular calcium concentrations. The dye, therefore, appears to be useful in excitatory cells or extracellular spaces with intracellular calcium concentrations high enough to saturate typical excitation ratio Ca2+ indicators such as Fura-2. Four new potential fluorescent probes for Ca super(2+) using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding affinities for Ca super(2+) determined. The most promising derivative for intracellular use, BTC, exhibits an excitation wavelength shift from 462 nm to 401 nm on binding Ca super(2+), with an emission wavelength of 530 nm. The quantum yield of this probe increases from 0.07 as the free indicator to 0.12 on binding Ca super(2+). BTC, loaded as its tetraacetoxymethyl ester (AM ester) into mouse myeloma P3X cells, responded only when cytoplasmic Ca super(2+) exceeded typical intracellular calcium concentrations. The dye, therefore, appears to be useful in excitatory cells or extracellular spaces with intracellular calcium concentrations high enough to saturate typical excitation ratio Ca super(2+) indicators such as Fura-2. Four new potential fluorescent probes for Ca 2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding affinities for Ca 2+ determined. The most promising derivative for intracellular use, BTC, exhibits an excitation wavelength shift from 462 nm to 401 nm on binding Ca 2+, with an emission wavelength of 530 nm. The quantum yield of this probe increases from 0.07 as the free indicator to 0.12 on binding Ca 2+. BTC, loaded as its tetraacetoxymethyl ester (AM ester) into mouse myeloma P3X cells, responded only when cytoplasmic Ca 2+ exceeded typical intracellular calcium concentrations. The dye, therefore, appears to be useful in excitatory cells or extracellular spaces with intracellular calcium concentrations high enough to saturate typical excitation ratio Ca 2+ indicators such as Fura-2. |
| Author | Marcus, E.M. Foukaraki, E. Iatridou, H. Kuhn, M.A. Katerinopoulos, H.E. Haugland, R.P. |
| Author_xml | – sequence: 1 givenname: H. surname: Iatridou fullname: Iatridou, H. organization: Department of Chemistry, University of Crete, Iraklion, Crete, Greece – sequence: 2 givenname: E. surname: Foukaraki fullname: Foukaraki, E. organization: Department of Chemistry, University of Crete, Iraklion, Crete, Greece – sequence: 3 givenname: M.A. surname: Kuhn fullname: Kuhn, M.A. organization: Molecular Probes Inc., Eugene, Oregon, USA – sequence: 4 givenname: E.M. surname: Marcus fullname: Marcus, E.M. organization: Molecular Probes Inc., Eugene, Oregon, USA – sequence: 5 givenname: R.P. surname: Haugland fullname: Haugland, R.P. organization: Molecular Probes Inc., Eugene, Oregon, USA – sequence: 6 givenname: H.E. surname: Katerinopoulos fullname: Katerinopoulos, H.E. organization: Department of Chemistry, University of Crete, Iraklion, Crete, Greece |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4132517$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/8149419$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkEtr3DAQgEVISTdp_kEKPpSSHNxqJFm2LikhJG0h0Et6FvJoRFT82Ep2Sv597eyyxxYGhmG-efCdsuNhHIixC-CfgIP-zEHJUoHml0ZdGc6rphRHbAOVFCUYA8dsc0DestOcf3HOjazhhJ00oIwCs2FfHp-o8PRM3bjtaZiKMRSuGOhPEVwfu5e1jsOUHFLXzZ1LBboO49wX2zS2lN-xN8F1mc73-Yz9vL97vP1WPvz4-v325qFE1ciplMKQaJXTOtSictq4JhisnSFstfdBh6CUlsZL4xBaRKg0rysUgqNHb-QZ-7jbu5z9PVOebB_z-pMbaJyzrbUSZon_gqCbRlWyWkC1AzGNOScKdpti79KLBW5XwXa1Z1d71ij7KtiKZez9fv_c9uQPQ3ujS__Dvu_yoiokN2DMB0yBFBXUC3a9w2iR9hwp2YyRBiQfE-Fk_Rj__cdf3eqX7Q |
| CODEN | CECADV |
| CitedBy_id | crossref_primary_10_1016_1011_1344_94_07052_P crossref_primary_10_1111_j_1469_7793_2001_00647_x crossref_primary_10_1063_1_1319859 crossref_primary_10_1152_physrev_1999_79_4_1089 crossref_primary_10_1016_0028_3908_95_00129_T crossref_primary_10_1016_S0040_4039_00_01662_2 crossref_primary_10_1016_S0143_4160_02_00035_0 crossref_primary_10_1016_S0143_4160_98_90126_9 crossref_primary_10_1038_nmeth_1663 crossref_primary_10_1002_cbic_200900214 crossref_primary_10_1016_S0143_4160_03_00022_8 crossref_primary_10_1021_ic900247r crossref_primary_10_1039_b811569a crossref_primary_10_1089_adt_2020_1011 crossref_primary_10_1002_chem_201101434 crossref_primary_10_1054_ceca_1999_0092 crossref_primary_10_1016_S0040_4039_03_01211_5 crossref_primary_10_1111_j_1469_7793_2001_t01_1_00757_x crossref_primary_10_1021_acs_biochem_1c00350 crossref_primary_10_1002_chem_201001665 crossref_primary_10_1523_JNEUROSCI_17_17_06669_1997 crossref_primary_10_1152_jn_01073_2003 crossref_primary_10_1016_j_ceca_2013_08_001 crossref_primary_10_1016_j_cell_2020_02_026 crossref_primary_10_1177_02611929211073038 crossref_primary_10_1021_ac0111107 crossref_primary_10_1038_aps_2017_75 crossref_primary_10_1002_0471142301_ns0711s04 crossref_primary_10_1016_0143_4160_95_90046_2 crossref_primary_10_1054_ceca_2001_0240 crossref_primary_10_1074_jbc_271_30_17751 |
| Cites_doi | 10.1039/jr9560001480 10.1016/S0040-4039(00)97487-2 10.1016/S0021-9258(18)83165-9 10.1002/cyto.990130705 10.1016/0143-4160(85)90040-5 10.1016/0166-2236(88)90192-0 10.1146/annurev.ne.12.030189.001303 10.1016/S0076-6879(89)72017-6 10.1038/290527a0 10.1146/annurev.ne.10.030187.003221 10.1016/0079-6107(82)90011-6 10.1016/S0021-9258(19)83641-4 10.1002/cber.19671000505 |
| ContentType | Journal Article |
| Copyright | 1994 1994 INIST-CNRS |
| Copyright_xml | – notice: 1994 – notice: 1994 INIST-CNRS |
| DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QP 7X8 |
| DOI | 10.1016/0143-4160(94)90058-2 |
| DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic Calcium & Calcified Tissue Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Anatomy & Physiology Biology |
| EISSN | 1532-1991 |
| EndPage | 198 |
| ExternalDocumentID | 10_1016_0143_4160_94_90058_2 8149419 4132517 0143416094900582 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M -~X .1- .FO .GJ .~1 0R~ 1B1 1P~ 1RT 1~. 1~5 29B 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 6J9 7-5 71M 8P~ 9JM AABNK AACTN AADPK AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXLA AAXUO ABCQJ ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AEVXI AFCTW AFFNX AFKWA AFRHN AFTJW AFXIZ AGHFR AGRDE AGUBO AGWIK AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV AJUYK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG COF CS3 DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HMK HMO HVGLF HZ~ IHE J1W KOM M29 M41 MO0 MOBAO N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SAE SCC SDF SDG SDP SES SEW SPCBC SSN SSU SSZ T5K UHS WUQ Z5R ZA5 ZGI ~G- 08R AALMO ABFLS ABPIF ABQIS ADALY IPNFZ IQODW AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QP 7X8 |
| ID | FETCH-LOGICAL-c483t-329e2b4a66f725a69a8f9c7a9ecb6ddf6ff44639d39ac1bcc156075c220cdcd93 |
| ISSN | 0143-4160 |
| IngestDate | Fri Aug 16 03:58:01 EDT 2024 Fri Aug 16 11:50:15 EDT 2024 Thu Sep 26 18:26:16 EDT 2024 Sat Sep 28 07:32:43 EDT 2024 Thu Nov 24 18:28:08 EST 2022 Fri Feb 23 02:20:33 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | Analysis method Calcium Probe characterization Spectral properties Intracellular Chemical synthesis Quantitative analysis |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c483t-329e2b4a66f725a69a8f9c7a9ecb6ddf6ff44639d39ac1bcc156075c220cdcd93 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
| PMID | 8149419 |
| PQID | 16884535 |
| PQPubID | 23462 |
| PageCount | 9 |
| ParticipantIDs | proquest_miscellaneous_76429429 proquest_miscellaneous_16884535 crossref_primary_10_1016_0143_4160_94_90058_2 pubmed_primary_8149419 pascalfrancis_primary_4132517 elsevier_sciencedirect_doi_10_1016_0143_4160_94_90058_2 |
| PublicationCentury | 1900 |
| PublicationDate | 1994-02-01 |
| PublicationDateYYYYMMDD | 1994-02-01 |
| PublicationDate_xml | – month: 02 year: 1994 text: 1994-02-01 day: 01 |
| PublicationDecade | 1990 |
| PublicationPlace | Oxford |
| PublicationPlace_xml | – name: Oxford – name: Netherlands |
| PublicationTitle | Cell calcium (Edinburgh) |
| PublicationTitleAlternate | Cell Calcium |
| PublicationYear | 1994 |
| Publisher | Elsevier India Pvt Ltd Elsevier |
| Publisher_xml | – name: Elsevier India Pvt Ltd – name: Elsevier |
| References | Tsien (BIB12) 1989; 12 Wanshoff, Malerne (BIB17) 1973 Tsien, Pozzan (BIB9) 1989; 172 Haugland (BIB14) 1992 Minta, Kao, Tsien (BIB22) 1989; 264 Lund-Johansen, Olews (BIB20) 1992; 13 Minta, Harootunian, Kao, Tsien (BIB10) 1987; 105 Demarinis, Katerinopoulos, Muirhead (BIB13) 1989 Tsien (BIB11) 1988; 11 Campbell (BIB1) 1983 Hille (BIB2) 1984 Rink, Pozzan (BIB8) 1985; 6 Papazoglou, Katerinopoulos, Fotakis, Iatridou, Foukaraki (BIB21) 1993; 1878 Blinks, Wier, Hess, Prendergast (BIB4) 1982; 40 Augustine, Charlton, Smith (BIB3) 1987; 10 Tsien, Rink (BIB5) 1983; Vol III Tsien (BIB6) 1981; 290 Inglish (BIB15) 1976; Vol. I Scheithauer, Mayer (BIB16) 1967; 100 Grynkiewicz, Poenie, Tsien (BIB7) 1985; 260 Hammer (BIB18) 1956 Corey, Carpino (BIB19) 1990; 31 Lund-Johansen (10.1016/0143-4160(94)90058-2_BIB20) 1992; 13 Demarinis (10.1016/0143-4160(94)90058-2_BIB13) 1989 Augustine (10.1016/0143-4160(94)90058-2_BIB3) 1987; 10 Tsien (10.1016/0143-4160(94)90058-2_BIB5) 1983; Vol III Tsien (10.1016/0143-4160(94)90058-2_BIB12) 1989; 12 Inglish (10.1016/0143-4160(94)90058-2_BIB15) 1976; Vol. I Grynkiewicz (10.1016/0143-4160(94)90058-2_BIB7) 1985; 260 Haugland (10.1016/0143-4160(94)90058-2_BIB14) 1992 Wanshoff (10.1016/0143-4160(94)90058-2_BIB17) 1973 Corey (10.1016/0143-4160(94)90058-2_BIB19) 1990; 31 Campbell (10.1016/0143-4160(94)90058-2_BIB1) 1983 Rink (10.1016/0143-4160(94)90058-2_BIB8) 1985; 6 Tsien (10.1016/0143-4160(94)90058-2_BIB9) 1989; 172 Minta (10.1016/0143-4160(94)90058-2_BIB10) 1987; 105 Tsien (10.1016/0143-4160(94)90058-2_BIB11) 1988; 11 Hammer (10.1016/0143-4160(94)90058-2_BIB18) 1956 Papazoglou (10.1016/0143-4160(94)90058-2_BIB21) 1993; 1878 Hille (10.1016/0143-4160(94)90058-2_BIB2) 1984 Minta (10.1016/0143-4160(94)90058-2_BIB22) 1989; 264 Scheithauer (10.1016/0143-4160(94)90058-2_BIB16) 1967; 100 Blinks (10.1016/0143-4160(94)90058-2_BIB4) 1982; 40 Tsien (10.1016/0143-4160(94)90058-2_BIB6) 1981; 290 |
| References_xml | – volume: Vol III start-page: 249 year: 1983 end-page: 312 ident: BIB5 article-title: Measurement of free Ca publication-title: Current Methods in Cellular Neurobiology contributor: fullname: Rink – volume: 12 start-page: 227 year: 1989 end-page: 253 ident: BIB12 article-title: Fluorescent probes of cell signalling publication-title: Annu. Rev. Neurosci. contributor: fullname: Tsien – year: 1989 ident: BIB13 article-title: Fluorescent intracellular calcium indicators contributor: fullname: Muirhead – volume: 1878 start-page: 57 year: 1993 end-page: 64 ident: BIB21 article-title: Laser induced fluorescence detection of cardiovascular calcified deposits via tetracarboxylate ion dye probing publication-title: Diagnostic and Therapeutic Cardiovascular Interventions III contributor: fullname: Foukaraki – volume: 11 start-page: 419 year: 1988 end-page: 424 ident: BIB11 article-title: Fluorescent measurement and photochemical manipulation of cytosolic free calcium publication-title: Trends Neurosci. contributor: fullname: Tsien – volume: 6 start-page: 133 year: 1985 end-page: 144 ident: BIB8 article-title: Using Quin-2 in cell suspensions publication-title: Cell Calcium contributor: fullname: Pozzan – year: 1992 ident: BIB14 publication-title: Handbook of Fluorescent Probes and Research Chemicals 1992–1994 contributor: fullname: Haugland – year: 1983 ident: BIB1 publication-title: Intracellular Calcium: Its Universal Role as Regulator contributor: fullname: Campbell – volume: 105 start-page: 89a year: 1987 ident: BIB10 article-title: New fluorescent indicators for intracellular sodium and calcium publication-title: J. Cell Biol. contributor: fullname: Tsien – start-page: 1480 year: 1956 end-page: 1498 ident: BIB18 article-title: Some chain substituted methincyanines and styryl dyes publication-title: J. Chem. Soc. contributor: fullname: Hammer – volume: 31 start-page: 3857 year: 1990 end-page: 3861 ident: BIB19 article-title: A new enantiospecific route to pseudopterocenes publication-title: Tetrahedron Lett. contributor: fullname: Carpino – volume: 40 start-page: 1 year: 1982 end-page: 114 ident: BIB4 article-title: Measurement of Ca publication-title: Progr. Biophys. Mol. Biol. contributor: fullname: Prendergast – volume: 260 start-page: 3440 year: 1985 end-page: 3450 ident: BIB7 article-title: A new generation of Ca publication-title: J. Biol. Chem. contributor: fullname: Tsien – volume: Vol. I start-page: 254 year: 1976 end-page: 256 ident: BIB15 article-title: Ethyl cyanoacetate publication-title: Org. Syn. Coll. contributor: fullname: Inglish – volume: 13 start-page: 693 year: 1992 end-page: 702 ident: BIB20 article-title: Signal transduction in monocytes and granulocytes measured by multiparameter flow cytometry publication-title: Cytometry contributor: fullname: Olews – year: 1984 ident: BIB2 publication-title: Ionic Channels of Excitable Membranes contributor: fullname: Hille – volume: 100 start-page: 1413 year: 1967 end-page: 1427 ident: BIB16 article-title: Synthese und reaktionen einenger thiomalonsaure diathyl ester und dithiocarbonsaureester publication-title: Chem. Ber. contributor: fullname: Mayer – volume: 264 start-page: 8171 year: 1989 end-page: 8178 ident: BIB22 article-title: Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores publication-title: J. Biol. Chem. contributor: fullname: Tsien – start-page: 573 year: 1973 end-page: 577 ident: BIB17 article-title: Zur synthese von Δ publication-title: Liebigs Ann. Chem. contributor: fullname: Malerne – volume: 10 start-page: 633 year: 1987 end-page: 693 ident: BIB3 article-title: Calcium action in synaptic transmitter release publication-title: Annu. Rev. Neurosci. contributor: fullname: Smith – volume: 290 start-page: 527 year: 1981 end-page: 528 ident: BIB6 article-title: A non disruptive technique for loading calcium buffers and indicators into cells publication-title: Nature contributor: fullname: Tsien – volume: 172 start-page: 230 year: 1989 end-page: 261 ident: BIB9 article-title: Measurement of cytosolic free Ca publication-title: Meth. Enzymol. contributor: fullname: Pozzan – volume: Vol. I start-page: 254 year: 1976 ident: 10.1016/0143-4160(94)90058-2_BIB15 article-title: Ethyl cyanoacetate publication-title: Org. Syn. Coll. contributor: fullname: Inglish – start-page: 1480 year: 1956 ident: 10.1016/0143-4160(94)90058-2_BIB18 article-title: Some chain substituted methincyanines and styryl dyes publication-title: J. Chem. Soc. doi: 10.1039/jr9560001480 contributor: fullname: Hammer – volume: 31 start-page: 3857 year: 1990 ident: 10.1016/0143-4160(94)90058-2_BIB19 article-title: A new enantiospecific route to pseudopterocenes publication-title: Tetrahedron Lett. doi: 10.1016/S0040-4039(00)97487-2 contributor: fullname: Corey – volume: 264 start-page: 8171 year: 1989 ident: 10.1016/0143-4160(94)90058-2_BIB22 article-title: Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)83165-9 contributor: fullname: Minta – volume: 13 start-page: 693 year: 1992 ident: 10.1016/0143-4160(94)90058-2_BIB20 article-title: Signal transduction in monocytes and granulocytes measured by multiparameter flow cytometry publication-title: Cytometry doi: 10.1002/cyto.990130705 contributor: fullname: Lund-Johansen – volume: 1878 start-page: 57 year: 1993 ident: 10.1016/0143-4160(94)90058-2_BIB21 article-title: Laser induced fluorescence detection of cardiovascular calcified deposits via tetracarboxylate ion dye probing contributor: fullname: Papazoglou – volume: 105 start-page: 89a year: 1987 ident: 10.1016/0143-4160(94)90058-2_BIB10 article-title: New fluorescent indicators for intracellular sodium and calcium publication-title: J. Cell Biol. contributor: fullname: Minta – year: 1984 ident: 10.1016/0143-4160(94)90058-2_BIB2 contributor: fullname: Hille – year: 1983 ident: 10.1016/0143-4160(94)90058-2_BIB1 contributor: fullname: Campbell – volume: 6 start-page: 133 year: 1985 ident: 10.1016/0143-4160(94)90058-2_BIB8 article-title: Using Quin-2 in cell suspensions publication-title: Cell Calcium doi: 10.1016/0143-4160(85)90040-5 contributor: fullname: Rink – year: 1989 ident: 10.1016/0143-4160(94)90058-2_BIB13 contributor: fullname: Demarinis – year: 1992 ident: 10.1016/0143-4160(94)90058-2_BIB14 contributor: fullname: Haugland – volume: 11 start-page: 419 year: 1988 ident: 10.1016/0143-4160(94)90058-2_BIB11 article-title: Fluorescent measurement and photochemical manipulation of cytosolic free calcium publication-title: Trends Neurosci. doi: 10.1016/0166-2236(88)90192-0 contributor: fullname: Tsien – volume: 12 start-page: 227 year: 1989 ident: 10.1016/0143-4160(94)90058-2_BIB12 article-title: Fluorescent probes of cell signalling publication-title: Annu. Rev. Neurosci. doi: 10.1146/annurev.ne.12.030189.001303 contributor: fullname: Tsien – volume: 172 start-page: 230 year: 1989 ident: 10.1016/0143-4160(94)90058-2_BIB9 article-title: Measurement of cytosolic free Ca2+ with quin-2: Practical aspects publication-title: Meth. Enzymol. doi: 10.1016/S0076-6879(89)72017-6 contributor: fullname: Tsien – volume: 290 start-page: 527 year: 1981 ident: 10.1016/0143-4160(94)90058-2_BIB6 article-title: A non disruptive technique for loading calcium buffers and indicators into cells publication-title: Nature doi: 10.1038/290527a0 contributor: fullname: Tsien – volume: 10 start-page: 633 year: 1987 ident: 10.1016/0143-4160(94)90058-2_BIB3 article-title: Calcium action in synaptic transmitter release publication-title: Annu. Rev. Neurosci. doi: 10.1146/annurev.ne.10.030187.003221 contributor: fullname: Augustine – volume: Vol III start-page: 249 year: 1983 ident: 10.1016/0143-4160(94)90058-2_BIB5 article-title: Measurement of free Ca2+ in cytoplasm contributor: fullname: Tsien – volume: 40 start-page: 1 year: 1982 ident: 10.1016/0143-4160(94)90058-2_BIB4 article-title: Measurement of Ca2+ concentrations in living cells publication-title: Progr. Biophys. Mol. Biol. doi: 10.1016/0079-6107(82)90011-6 contributor: fullname: Blinks – volume: 260 start-page: 3440 year: 1985 ident: 10.1016/0143-4160(94)90058-2_BIB7 article-title: A new generation of Ca2+ indicators with greatly improved fluorescence properties publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)83641-4 contributor: fullname: Grynkiewicz – volume: 100 start-page: 1413 year: 1967 ident: 10.1016/0143-4160(94)90058-2_BIB16 article-title: Synthese und reaktionen einenger thiomalonsaure diathyl ester und dithiocarbonsaureester publication-title: Chem. Ber. doi: 10.1002/cber.19671000505 contributor: fullname: Scheithauer – start-page: 573 year: 1973 ident: 10.1016/0143-4160(94)90058-2_BIB17 article-title: Zur synthese von Δ2-thiazolinyl-Δ2-Oxazolinyl-benzoxazolyl- und perimidinyl-2-essigsaure-athylestern publication-title: Liebigs Ann. Chem. contributor: fullname: Wanshoff |
| SSID | ssj0009371 |
| Score | 1.6114467 |
| Snippet | Four new potential fluorescent probes for Ca
2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding... Four new potential fluorescent probes for Ca2+ using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and binding... Four new potential fluorescent probes for Ca super(2+) using the coumarin moiety as a fluorophore have been synthesized and their spectral properties and... |
| SourceID | proquest crossref pubmed pascalfrancis elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 190 |
| SubjectTerms | Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Calcium - analysis Coumarins Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology Mice |
| Title | The development of a new family of intracellular calcium probes |
| URI | https://dx.doi.org/10.1016/0143-4160(94)90058-2 https://www.ncbi.nlm.nih.gov/pubmed/8149419 https://search.proquest.com/docview/16884535 https://search.proquest.com/docview/76429429 |
| Volume | 15 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fb9MwELagE2gSQrAxrcDAD4DgIdD4RxI_oYJaDboOCbWib5btJAKhNVOXPMBfz7m200ZQDZCqKE0aR727-D5f7rtD6Bm40UJlykRJZotqg0-LFBU6YjlPUzWg4BMtUXh6npzO2ccFX4Qe7p5dUuvX5ucfeSX_o1U4Bnq1LNl_0Gw7KByAfdAvbEHDsP1rHeebrB_HdQScHMIWrhzEStno_DrdFBRivjUXNitL--TBUKXAxvDCaUCdI_Bp_g1MGyr4YMv551XT4TSMq-a7WinX_bqlNUyary5JeBMrncIj1Tjygw_B5p59x0J-8lb8kUaA4QadCZRvGQrZmg1j1wnUO9bYtZv-bc524YN2ZPiLgj23XnLAM0-N7JTJPv8kx_OzMzkbLWY30R5JBec9tDecfP4y2dRbpqlrRelHDazJOHnTHnsp2Ct_l12o5M6lugLZl67Jye5VyBqNzO6hu34ZgYfOJu6jG8XyAB0Ol6quLn7gF3id2Lt-Y3KAbr0Le7enPpPiEL0F28FbtoOrEisMtoOd7djvHdvB3jiws50HaD4ezd6fRr6ZRmRYRuuIElEQzVSSlCnhKhEqK4VJlSiMTvK8TMqSMYCrORXKxNoYS7FPuSFkYHKTC3qEestqWRwjrInmJINTtBAsJ1SXvIBVL1EAp3Wskz6KgjDlpauZIkMyoRW-tMKXgsm18CXpozRIXHrc5_CcBBO55sqTjoLa2wE0s7X4-uhpUJiEadNKTC2LqrmScZJljFO--xcprMwFfProyGm6HTyLmWCxeHjtpY_Q_uYheox69aopTgDD1vqJt9df9XuU_g |
| link.rule.ids | 315,786,790,27955,27956 |
| linkProvider | Library Specific Holdings |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+development+of+a+new+family+of+intracellular+calcium+probes&rft.jtitle=Cell+calcium+%28Edinburgh%29&rft.au=Iatridou%2C+H&rft.au=Foukaraki%2C+E&rft.au=Kuhn%2C+M+A&rft.au=Marcus%2C+E+M&rft.date=1994-02-01&rft.issn=0143-4160&rft.volume=15&rft.issue=2&rft.spage=190&rft.epage=198&rft_id=info:doi/10.1016%2F0143-4160%2894%2990058-2&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0143-4160&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0143-4160&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0143-4160&client=summon |