An optimized protocol for stepwise optimization of real-time RT-PCR analysis - Zhao, Fangzhou; Maren, Nathan A.; Kosentka, Pawel Z.; Liao, Ying-Yu; Lu, Hongyan; Duduit, James R.; Huang, Debao; Ashrafi, Hamid; Zhao, Tuanjie; Huerta, Alejandra I.; Ranney, Thomas G.; Liu, Wusheng (Horticulture research, 2021)
Keywords: 631/1647/2017; 631/337/572; 631/449/1659; Agriculture; Biomedical and Life Sciences; Case studies; Complementary DNA; Computer applications; Data analysis; Developmental stages; Ecology; Efficiency; Genes; Genomes; Homology; Life Sciences; Method; Nucleotide sequence; Nucleotides; Optimization; Parameter sensitivity; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; Polymerase chain reaction; Real time; Reverse transcription; Single-nucleotide polymorphism; Software; Soybeans