Reactivation of silenced transgene expression in mouse liver by rapid, large-volume injection of isotonic solution

• Published in: Human gene therapy Vol. 19; no. 10; p. 1009
• Main Authors: Nishikawa, Makiya, Nakayama, Ayumi, Takahashi, Yuki, Fukuhara, Yasushi, Takakura, Yoshinobu
• Format: Journal Article
• Language: English
• Published: United States 01.10.2008
• Subjects: Animals; Gene Expression Profiling; Gene Silencing - drug effects; Genes, fos - physiology; Genes, jun - physiology; Humans; Isotonic Solutions - pharmacology; Liver - metabolism; Male; Mice; Mice, Inbred ICR; NF-kappa B - metabolism; Oligonucleotide Array Sequence Analysis; Plasmids - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factor AP-1 - metabolism; Transgenes

Rapid, large-volume injection, or so-called hydrodynamic injection, of naked plasmid DNA gives high transgene expression in mouse liver, and this method has been applied to liver-directed gene transfer in humans with slight modifications. To prove that injection-induced biological changes are involv...