Chemical repair of base lesions, AP-sites, and strand breaks on plasmid DNA in dilute aqueous solution by ascorbic acid

• Published in: Biochemical and biophysical research communications Vol. 434; no. 2; pp. 341 - 345
• Main Authors: Hata, Kuniki, Urushibara, Ayumi, Yamashita, Shinichi, Shikazono, Naoya, Yokoya, Akinari, Katsumura, Yosuke
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.05.2013
• Subjects: ANTIOXIDANTS; Antioxidants - chemistry; AQUEOUS SOLUTIONS; ASCORBIC ACID; Ascorbic Acid - chemistry; BIOLOGICAL REPAIR; Chemical repair; CONCENTRATION RATIO; DNA; DNA Breaks, Single-Stranded - drug effects; DNA Repair; DNA, Bacterial - drug effects; DNA, Bacterial - genetics; DNA, Bacterial - radiation effects; Dose-Response Relationship, Drug; Electrophoresis, Agar Gel; Escherichia coli - genetics; Gamma Rays; GELS; IRRADIATION; Nucleic Acid Conformation; Plasmids - drug effects; Plasmids - genetics; Plasmids - radiation effects; PRECURSOR; Radiation damage; RADIATION PROTECTION; RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; Radiation-Protective Agents - chemistry; RADIOLYSIS; Solutions - metabolism; STRAND BREAKS; THERMODYNAMIC ACTIVITY; Water - metabolism; Antioxidants; Ascorbic acid; Chemical repair; Radiation damage

•We report a novel mechanism of radiation protection of DNA by chemical activity of ascorbic acid.•The “chemical repair” of DNA damage was revealed using biochemical assay and chemical kinetics analysis.•We found that ascorbic acid significantly repairs precursors of nucleobase lesions and abasic si...