Apoptotic hair cell death after transient cochlear ischemia in gerbils

The mechanisms of cochlear hair cell death following exposure to transient inner ear ischemia were investigated in gerbils histologically. The animals were subjected to ischemic insult by occluding both vertebral arteries for 15 min. Hoechst 33342 nuclear staining showed that inner hair cells (IHCs)...

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Published inNeuroreport Vol. 13; no. 18; p. 2459
Main Authors Taniguchi, Masafumi, Hakuba, Nobuhiro, Koga, Kenichiro, Watanabe, Futoshi, Hyodo, Jun, Gyo, Kiyofumi
Format Journal Article
LanguageEnglish
Published England 20.12.2002
Subjects
Animals
Apoptosis
Benzimidazoles
Cricetinae
Fluorescent Dyes
Hair Cells, Auditory - pathology
Hair Cells, Auditory - ultrastructure
In Situ Nick-End Labeling
Male
Microscopy, Electron
Reperfusion Injury - pathology
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Summary:The mechanisms of cochlear hair cell death following exposure to transient inner ear ischemia were investigated in gerbils histologically. The animals were subjected to ischemic insult by occluding both vertebral arteries for 15 min. Hoechst 33342 nuclear staining showed that inner hair cells (IHCs) underwent sporadic degeneration via nuclear condensation, which peaked 12 hours after the ischemia. Furthermore, nuclear DNA fragmentation was noted by the terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling method. Transmission electron microscopy revealed morphological changes in the IHCs characteristic of apoptosis, including karyopyknosis, chromatin condensation. These findings suggest that apoptotic cell death is the major process in hair cell degeneration in this animal model.
ISSN:0959-4965
DOI:10.1097/00001756-200212200-00017