Buildup from birth onward of short telomeres in human hematopoietic cells

• Published in: Aging cell Vol. 22; no. 6; pp. e13844 - n/a
• Main Authors: Lai, Tsung‐Po, Verhulst, Simon, Savage, Sharon A., Gadalla, Shahinaz M., Benetos, Athanase, Toupance, Simon, Factor‐Litvak, Pam, Susser, Ezra, Aviv, Abraham
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.06.2023; Wiley Open Access; John Wiley and Sons Inc
• Subjects: Age; Aged, 80 and over; Aging; Biology; Cell Division; Chromosomes; Dyskeratosis; Enzymes; Epidemiology; Female; Hematopoietic stem cells; Humans; Leukocytes; Life Sciences; Life span; lifetime; Longevity; Male; Males; Measurement techniques; Polymerase chain reaction; Senescence; sex; Sex differences; Somatic cells; Southern blotting; subtelomeric region; Telomerase; Telomere - genetics; telomere biology disorders; Telomere Shortening; Telomeres; terminal restriction fragments; TeSLA; Yeast; terminal restriction fragments; sex; telomeres; lifetime; TeSLA; subtelomeric region; Southern blotting; telomere biology disorders; age; fluorescent in situ hybridization; single gene product SB; telomere length TRFs; LTL; SBmTL; measured by TeSLA TeSmTL; single gene product; TRFs; dyskeratosis congenita-related telomere biology disorders FISH; TeSmTL; bp; Southern blotting SBmTL; SB; kilobase; S; SNP; T; proportion of telomeres less than 3 kb; DC/TBD; kilobase LTL; kb; amplified telomere product; mean TL using TeSLA; qPCR; hematopoietic cell kb; single-nucleotide polymorphism; telomere length; single-nucleotide polymorphism T; measured by TeSLA; amplified telomere product TeS3kb; TeS3kb; mean TL using SB; quantitative polymerase chain reaction S; mean TL using TeSLA TL; quantitative polymerase chain reaction; base pairs DC/TBD; mean TL using SB SNP; base pairs; hematopoietic cell; dyskeratosis congenita-related telomere biology disorders; fluorescent in situ hybridization HC; leukocyte telomere length qPCR; TL; FISH; HC; leukocyte telomere length

Telomere length (TL) limits somatic cell replication. However, the shortest among the telomeres in each nucleus, not mean TL, is thought to induce replicative senescence. Researchers have relied on Southern blotting (SB), and techniques calibrated by SB, for precise measurements of TL in epidemiolog...