Transcriptional Pausing in Vivo: A Nascent RNA Hairpin Restricts Lateral Movements of RNA Polymerase in Both Forward and Reverse Directions

• Published in: Journal of molecular biology Vol. 351; no. 1; pp. 39 - 51
• Main Authors: Toulmé, Francine, Mosrin-Huaman, Christine, Artsimovitch, Irina, Rahmouni, A. Rachid
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 05.08.2005; Elsevier
• Subjects: Base Sequence; DNA Footprinting; DNA-Directed RNA Polymerases - metabolism; Escherichia coli; Escherichia coli - genetics; hairpin-dependent pausing; initially transcribed sequence; Nucleic Acid Conformation; RNA - chemistry; RNA - genetics; RNA - physiology; RNA polymerase backtracking; transcription regulation; Transcription, Genetic; transcriptional pausing; transcription regulation; CAA; transcriptional pausing; hairpin-dependent pausing; RNA polymerase backtracking; initially transcribed sequence; RNAP; TC

Transcriptional pausing by RNA polymerase has been the subject of extensive investigations in vitro, yet little is known about its occurrence and significance in vivo. The transient nature of the pausing events makes them difficult to observe inside the cell, whereas their studies in vitro by classi...