Bacterial Skin Microbiota of Seabass from Aegean Fish Farms and Antibiotic Susceptibility of Psychrotrophic Pseudomonas

• Published in: Foods Vol. 12; no. 10; p. 1956
• Main Authors: Aydin, Ali, Sudagidan, Mert, Mamatova, Zhanylbubu, Yurt, Mediha Nur Zafer, Ozalp, Veli Cengiz, Zornu, Jacob, Tavornpanich, Saraya, Brun, Edgar
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.05.2023; MDPI
• Subjects: Amikacin; Aminoglycosides; Antibiotic resistance; Antibiotics; Aquaculture; Bacteria; Carbapenems; Ciprofloxacin; Farms; Fish; Fish farms; Fluoroquinolones; Food; Food science; Genetic testing; Gentamicin; Imipenem; Laboratories; Levofloxacin; Meropenem; Microbiota; Microorganisms; Next-generation sequencing; Norfloxacin; Phenols; Piperacillin; Piperacillin-tazobactam; Pseudomonas; seabass; Seafood; Skin; Strains (organisms); Susceptibility; Tazobactam; Tetracyclines; Tobramycin; Greece; Istanbul Turkey; Turkey; United Kingdom > UK; United States > US; Aegean Sea; Germany; microbiota; Pseudomonas; antibiotic resistance; seabass; fish farms
• DOI: 10.3390/foods12101956
• ISSN: 2304-8158

Farming seabass ( ) is an essential activity in the Mediterranean basin including the Aegean Sea. The main seabass producer is Turkey accounting for 155,151 tons of production in 2021. In this study, skin swabs of seabass farmed in the Aegean Sea were analysed with regard to the isolation and identification of . Bacterial microbiota of skin samples ( = 96) from 12 fish farms were investigated using next-generation sequencing (NGS) and metabarcoding analysis. The results demonstrated that Proteobacteria was the dominant bacterial phylum in all samples. At the species level, was identified in all samples. , and were identified using conventional methods and a total of 46 viable (48% of all NGS+) were isolated in seabass swab samples. Additionally, antibiotic susceptibility was determined according to standards of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) in psychrotrophic . strains were tested for susceptibility to 11 antibiotics (piperacillin-tazobactam, gentamicin, tobramycin, amikacin, doripenem, meropenem, imipenem, levofloxacin, ciprofloxacin, norfloxacin, and tetracycline) from five different groups of antibiotics (penicillins, aminoglycosides, carbapenems, fluoroquinolones, and tetracyclines). The antibiotics chosen were not specifically linked to usage by the aquaculture industry. According to the EUCAST and CLSI, three and two strains were found to be resistant to doripenem and imipenem (E-test), respectively. All strains were susceptible to piperacillin-tazobactam, amikacin, levofloxacin, and tetracycline. Our data provide insight into different bacteria that are prevalent in the skin microbiota of seabass sampled from the Aegean Sea in Turkey, and into the antibiotic resistance of psychrotrophic spp.