Generation and Phenotypic Analysis of a Transgenic Line of Rabbits Secreting Active Recombinant Human Erythropoietin in the Milk

• Published in: Transgenic research Vol. 13; no. 5; pp. 487 - 498
• Main Authors: Mikus, Tom s, Poplstein, Martin, Sedl kov, Jirina, Landa, Vladim r, Jen kov, Gabriela, Trefil, Pavel, Lidick, Jan, Mal, Petr
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.10.2004; Springer Nature B.V
• Subjects: Animals; Animals, Genetically Modified - genetics; Base Sequence; Biological and medical sciences; Biotechnology; Erythropoietin - secretion; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - genetics; Genetic engineering; Genetic technics; Humans; Isotope Labeling; Lactation - metabolism; Male; Mammary Glands, Animal - secretion; Methods. Procedures. Technologies; Milk; Milk - secretion; Milk Proteins - genetics; Molecular Sequence Data; Proteins; Rabbits - genetics; Recombinant Proteins; Thymidine - metabolism; Transgenic animals and transgenic plants; Human; Erythropoietin; Secretion; human erythropoietin; Rabbit; Lagomorpha; transgenic rabbit; Vertebrata; Mammalia; rabbit WAP; Recombinant protein; Transgenesis; Milk

Production of recombinant human erythropoietin (rhEPO) for therapeutic purposes relies on its expression in selected clones of transfected mammalian cells. Alternatively, this glycoprotein can be produced by targeted secretion into the body fluid of transgenic mammals. Here, we report on the generat...