Colony-Stimulating Factor (CSF) Radioimmunoassay: Detection of a CSF Subclass Stimulating Macrophage Production
Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified125I-labeled murine L cell CSF has been used to develop a radioimmunoassay (RIA) that detects a subclass of CSFs that stimulates macrophage production. Murine C...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 76; no. 6; pp. 2969 - 2973 |
---|---|
Main Author | |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences of the United States of America
01.06.1979
National Acad Sciences |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified125I-labeled murine L cell CSF has been used to develop a radioimmunoassay (RIA) that detects a subclass of CSFs that stimulates macrophage production. Murine CSF preparations that contain this subclass of CSF compete for all of the CSF binding sites on anti-L cell CSF antibody. With the exception of mouse serum, which can contain inhibitors of the bioassay, there is complete correspondence between activities determined by RIA and those determined by bioassay. The RIA is slightly more sensitive than the bioassay, detecting approximately 0.3 fmol of purified L cell CSF. It can also detect this subclass of CSF in chickens, rats, and humans. In the mouse, the subclass is distinguished from other CSFs by a murine cell bioassay dose-response curve in which 90% of the response occurs over a 10-fold (rather than a 100-fold) increase in concentration, by stimulating the formation of colonies containing a high proportion of mononuclear (rather than granulocytic) cells, and by certain physical characteristics. |
---|---|
AbstractList | Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified 125I-labeled murine L cell CSF has been used to develop a radioimmunoassay (RIA) that detects a subclass of CSFs that stimulates macrophage production. Murine CSF preparations that contain this subclass of CSF complete for all of the CSF binding sites on anti-L cell CSF antibody. With the exception of mouse serum, which can contain inhibitors of the bioassay, there is complete correspondence between activities determined by RIA and those determined by bioassay. The RIA is slightly more sensitive than the bioassay, detecting approximately 0.3 fmol of purified L cell CSF. It also detect this subclass of CSF in chickens, rats, and humans. In the mouse, the subclass is distinguished from other CSFs by a murine cell bioassay dose-response curve in which 90% of the response occurs over a 10-fold (rather than a 100-fold) increase in concentration, by stimulating the formation of colonies containing a high proportion of mononuclear (rather than granulocytic) cells, and by certain physical characteristics. Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified125I-labeled murine L cell CSF has been used to develop a radioimmunoassay (RIA) that detects a subclass of CSFs that stimulates macrophage production. Murine CSF preparations that contain this subclass of CSF compete for all of the CSF binding sites on anti-L cell CSF antibody. With the exception of mouse serum, which can contain inhibitors of the bioassay, there is complete correspondence between activities determined by RIA and those determined by bioassay. The RIA is slightly more sensitive than the bioassay, detecting approximately 0.3 fmol of purified L cell CSF. It can also detect this subclass of CSF in chickens, rats, and humans. In the mouse, the subclass is distinguished from other CSFs by a murine cell bioassay dose-response curve in which 90% of the response occurs over a 10-fold (rather than a 100-fold) increase in concentration, by stimulating the formation of colonies containing a high proportion of mononuclear (rather than granulocytic) cells, and by certain physical characteristics. |
Author | Stanley, E. Richard |
Author_xml | – sequence: 1 givenname: E. Richard surname: Stanley fullname: Stanley, E. Richard |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/313570$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kUuP0zAURi00PDoDWxYIJK9GsEjwK06MxGJUKCANAlFYW65z0_EosUvsjOi_x6WlKhvkhRffOdfX-s7RmQ8eEHpKSUlJzV9vvIllLUtZMiXVPTSjRNFCCkXO0IwQVheNYOIROo_xlhCiqoY8RA845VVNZijMQx_8tlgmN0y9Sc6v8cLYFEb8cr5cvMLfTOuCG4bJBxOj2b7B7yCBTS54HDpscKbwclrZPsf4dMxnY8ewuTFrwF_H0E5_nMfofmf6CE8O9wX6sXj_ff6xuP7y4dP86rqwomGpUKylVinKgULHbVOxyhgCHFTLO2JXNbOVqBQIIgRvgBjbrkxNhZLQMQuSX6C3-7mbaTVAa8Gn0fR6M7rBjFsdjNP_Jt7d6HW407zhNWfZvzz4Y_g5QUx6cNFC3xsPYYq6FjKfSmSw3IP5szGO0B3foETvCtK7gnQttdS7grLw_HSzI75vJMcvDvFO-xue6pf_y3U39X2CXymDz_bgbcxtHkmpGiX5bxJLsUE |
CitedBy_id | crossref_primary_10_1016_0021_9673_94_80312_9 crossref_primary_10_1189_jlb_0506304 crossref_primary_10_1002_jcp_1041550209 crossref_primary_10_1007_BF01806155 crossref_primary_10_1002_jss_400140403 crossref_primary_10_1111_j_1471_4159_1992_tb08366_x crossref_primary_10_1002_jcb_240210206 crossref_primary_10_1017_S0962279900000454 crossref_primary_10_1002_jcb_240210403 crossref_primary_10_1002_jcp_1041130412 crossref_primary_10_1016_j_tcb_2004_09_016 crossref_primary_10_1128_mcb_7_2_664_671_1987 crossref_primary_10_1002_jcp_1041220210 crossref_primary_10_1002_path_1711410316 crossref_primary_10_1182_blood_2003_08_2739 crossref_primary_10_3109_08977199109000273 crossref_primary_10_1182_blood_V99_1_111 crossref_primary_10_1007_BF02369893 crossref_primary_10_1002_1097_0142_20011201_92_11_2837__AID_CNCR10093_3_0_CO_2_5 crossref_primary_10_1002_jcb_240310303 crossref_primary_10_1002_eji_1830221012 crossref_primary_10_1002_stem_5530040104 crossref_primary_10_1007_BF01959758 crossref_primary_10_1002_jcp_1041060314 crossref_primary_10_3109_10428199409049662 crossref_primary_10_1359_jbmr_2001_16_2_361 crossref_primary_10_1016_S0015_0282_01_02735_2 crossref_primary_10_1189_jlb_1209822 crossref_primary_10_1016_S0308_2261_21_00427_6 crossref_primary_10_1016_j_fertnstert_2012_02_007 crossref_primary_10_1002_jcb_240400110 crossref_primary_10_1111_j_1365_2141_1992_tb04557_x crossref_primary_10_1002_jcp_1041030309 crossref_primary_10_1111_j_1749_6632_1989_tb22417_x crossref_primary_10_4049_jimmunol_177_6_4055 crossref_primary_10_1002_jcp_1041100108 crossref_primary_10_1182_blood_V98_1_74 crossref_primary_10_1016_S0301_472X_00_00512_9 crossref_primary_10_1080_09553008514550811 crossref_primary_10_1002_jcp_1041020102 crossref_primary_10_1002_jcp_1041300316 crossref_primary_10_1002_jcp_1041020303 crossref_primary_10_1242_dev_120_6_1357 crossref_primary_10_1002__SICI_1097_4652_199808_176_2_323__AID_JCP10_3_0_CO_2_4 crossref_primary_10_1002_jcp_1041220305 crossref_primary_10_3109_10428199609054784 crossref_primary_10_1002_jcp_1041160111 crossref_primary_10_1182_blood_2005_05_1822 crossref_primary_10_1002_jcp_1041220306 crossref_primary_10_1016_S0022_1759_83_80024_6 crossref_primary_10_1138_20100474 crossref_primary_10_1002_jcp_1041120215 crossref_primary_10_1038_298075a0 crossref_primary_10_1002_jcb_240310403 crossref_primary_10_1016_S0021_9258_18_45861_9 crossref_primary_10_3109_00365519509090576 crossref_primary_10_1016_S0021_9258_18_33500_2 crossref_primary_10_1016_S0171_2985_88_80050_0 crossref_primary_10_1189_jlb_0909624 crossref_primary_10_1002_jcp_1041150310 crossref_primary_10_1002_stem_5530040204 crossref_primary_10_1002_jcp_1041150111 crossref_primary_10_1002_jcp_1041240219 crossref_primary_10_1016_S0021_9258_17_36358_5 crossref_primary_10_1038_287228a0 crossref_primary_10_1002_jcp_1041170106 crossref_primary_10_1002_jcp_1041040309 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1073/pnas.76.6.2969 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE CrossRef MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) |
EISSN | 1091-6490 |
EndPage | 2973 |
ExternalDocumentID | 10_1073_pnas_76_6_2969 313570 76_6_2969 69896 |
Genre | Journal Article Comparative Study |
GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 123 29P 2AX 2FS 2WC 4.4 53G 5RE 5VS 6TJ 79B 85S AACGO AAFWJ AANCE ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACIWK ACNCT ACPRK ADULT ADZLD AENEX AEUPB AEXZC AFDAS AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM ASUFR AS~ CS3 D0L DCCCD DIK DNJUQ DOOOF DU5 DWIUU E3Z EBS EJD F20 F5P FRP GX1 HGD HH5 HQ3 HTVGU HYE JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST KQ8 L7B LU7 MVM N9A NEJ N~3 O9- OK1 P-O PNE PQQKQ R.V RHF RHI RNA RNS RPM RXW SA0 SJN TN5 UKR VOH VQA W8F WH7 WHG WOQ WOW X7M XSW ZA5 ZCA ZCG ~02 ~KM - 02 08R 0R 1AW 55 AAPBV ABFLS ABPTK ADACO AJYGW AS DZ GJ KM OHM PQEST X XHC 3O- 692 AAYJJ ACKIV ADACV BKOMP CGR CUY CVF ECM EIF H13 IPSME NHB NPM TAE Y6R YBH YKV YSK AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-c482t-92d1c9913e1ef3c8525aa0e3e9d3f0cb72c5459e404438e0acdba71496ef2ce63 |
IEDL.DBID | RPM |
ISSN | 0027-8424 |
IngestDate | Tue Sep 17 21:21:36 EDT 2024 Fri Aug 16 22:54:05 EDT 2024 Fri Aug 23 01:09:54 EDT 2024 Sat Sep 28 08:38:17 EDT 2024 Wed Nov 11 00:30:12 EST 2020 Thu May 30 08:53:00 EDT 2019 Fri Feb 02 07:04:46 EST 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c482t-92d1c9913e1ef3c8525aa0e3e9d3f0cb72c5459e404438e0acdba71496ef2ce63 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://doi.org/10.1073/pnas.76.6.2969 |
PMID | 313570 |
PQID | 74646454 |
PQPubID | 23479 |
PageCount | 5 |
ParticipantIDs | crossref_primary_10_1073_pnas_76_6_2969 jstor_primary_69896 pnas_primary_76_6_2969 proquest_miscellaneous_74646454 pubmed_primary_313570 pnas_primary_76_6_2969_fulltext pubmedcentral_primary_oai_pubmedcentral_nih_gov_383732 |
ProviderPackageCode | RNA PNE |
PublicationCentury | 1900 |
PublicationDate | 19790601 |
PublicationDateYYYYMMDD | 1979-06-01 |
PublicationDate_xml | – month: 6 year: 1979 text: 19790601 day: 1 |
PublicationDecade | 1970 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
PublicationTitleAlternate | Proc Natl Acad Sci U S A |
PublicationYear | 1979 |
Publisher | National Academy of Sciences of the United States of America National Acad Sciences |
Publisher_xml | – name: National Academy of Sciences of the United States of America – name: National Acad Sciences |
SSID | ssj0009580 |
Score | 1.482532 |
Snippet | Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified125I-labeled murine... Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified 125I-labeled... |
SourceID | pubmedcentral proquest crossref pubmed pnas jstor |
SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
StartPage | 2969 |
SubjectTerms | Animals Antiserum Bioassay Biological Assay Colony-Stimulating Factors - analysis Colony-Stimulating Factors - physiology Cultured cells Dose response relationship Embryonic cells Endotoxins Humans Immune Sera L cells L Cells (Cell Line) - physiology Lungs Macrophages Macrophages - physiology Mice Mice, Inbred C57BL Radioimmunoassay - methods Rats |
Title | Colony-Stimulating Factor (CSF) Radioimmunoassay: Detection of a CSF Subclass Stimulating Macrophage Production |
URI | https://www.jstor.org/stable/69896 http://www.pnas.org/content/76/6/2969.abstract https://www.ncbi.nlm.nih.gov/pubmed/313570 https://search.proquest.com/docview/74646454 https://pubmed.ncbi.nlm.nih.gov/PMC383732 |
Volume | 76 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwELVoxaEXREsrAqX4gER7SDaxHTvmhlasCqgICSr1Fjn-oCuxzqq7e-i_79hOVlvUE9d4nESesf0sv3mD0IeQnAkbT51LbpqcOe7yxlidNwFcW-eYEyFR-OoHv7xm327qmyEpbDXQKr3u5oX_uyj8_DZyK5cLPRl5YpOfV9NwqqJksof2BKXjCX0rtNuktBMCqy8jbBRqFHSy9GpVCF7wgkguD9BzWtE6VCne2ZESKTEonYLxU6jzX_Lkzm40e4leDDASf06_e4ieWX-EDoeJusLng5r0xSvUT2F58_c5TOVFLNXl_-BUZAefT3_NLvCdMvN-HtJEegDS6v4TNnYdCVoe9w4rDFZ4tel0wNl49zULFep_3cKKhJdJOBb6HKPr2Zff08t8qLKQa9aQdS6JqTSgRGor66hualIrVVpqpaGu1J0gGlCWtKxkjDa2VNp0SsDBClxJtOX0BO373tvXCFNVKdWZppOqZkJpxUxZulI4wBi6Y3WGPo4j3S6TmEYbL8EFbcN4t4K3vA3OydBRdMTWLNS55Bk6iWbjwx3z9083tG6g0ASLwZUtTKBwK6K87TfwTcajrFmGjpNjt29J4ZEh_sjh2-YgzP24BeI1CnSn-Hzzvx3fooNKCpkIaadof323se8A-qy7MwD9X7-fxYh_ADYCB5Q |
link.rule.ids | 230,315,733,786,790,891,27957,27958,53827,53829 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Bb9MwFH6CgcQuGxubyNiYD0hsh6SJ7dgJt6miKrBOSKzSbpHj2KyCOtWaHsavx46Tqpt2gWv87Mjy8_Nn-XvfA_jgkjPtwZOGOauykGqmw6xSMswcuFZaU81dovDkio2n9OtNetMlhS07WqWR5Swyv-eRmd223MrFXA56ntjg-2ToblUED57DC7tdMe_v6Gup3cwnnmAbfymmvVQjJ4OFEcuIs4hFOGf5NrwkCUldneKNM8nTEp3WqTV-Cnc-pk9unEejXZj2M_E0lF_Rqikj-eeRyOO_TvU17HQAFV341j14psw-7HUhYInOOp3q8zdQD23gNPehDRLztgiY-Yl8-R50NvwxOkd3oprVM5eAUluILu4_oUo1LfXLoFojgawVWq5K6RA82hxmLlxlsVsb69DCS9LaPgcwHX2-Ho7Drn5DKGmGmzDHVSIt_iQqUZrILMWpELEiKq-IjmXJsbT4LVc0ppRkKhayKgW3VzbrJFgqRg5hy9RGvQVERCJEWWVlLlLKhRS0imMdc23RiyxpGsDHfgWLhZfpKNrndU4Kt44FZwUr3KIHsN8u8NrMVdBkARy2Zv3HDfPTpxsK3ZFznEXnIoXdmu69RRhVr-w_KWsF0wI48A6zHsW7XQDsgSOtm53k98MW6x6t9Ld3h6P_7XgKr8bXk8vi8svVt3ewneQ897S3Y9hq7lbqxAKspnzf7qe_zfcoXA |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB5RWlVcaKGghj7woVLhkJftOElv1bYr-gAhtUj0FDmOXVbtOhGbPdBfXz-S1YI4cY3HjiyPx5_lb74BeGeTM83Bk4Ula4qQKqbCopEiLCy4lkpRldtE4dMzdnJBv15mlxtQjLkwjrQv6lmk_84jPbty3MpuLuKRJxafn07srYrguGtU_Agemy2Ly_GevpLbLXzyCTYxmGI6yjXmJO40X0Q5i1iES1ZuwROSkszWKl47lzw10eqdGuP7sOddCuXamTR9Br_G2Xgqyp9o2deR-HdH6PEh030O2wNQRR-9xQ5sSL0LO0MoWKCjQa_6-AW0ExNA9U1ogsXcFQPTv5Ev44OOJj-mx-iaN7N2ZhNRWgPV-c0H1MjeUcA0ahXiyFihxbIWFsmj9WHm3FYYuzIxD3Vemtb02YOL6eefk5NwqOMQClrgPixxkwqDQ4lMpSKiyHDGeSKJLBuiElHnWBgcV0qaUEoKmXDR1Dw3VzfjLFhIRvZhU7davgREeMp53RR1yTOac8FpkyQqyZVBMaKmWQDvx1WsOi_XUbln9pxUdi2rnFWssgsfwK5b5JWZraTJAth3ZuPHNfPD-xsqNZB0rMXgJpXZovbdhWvZLs0_KXPCaQHseadZjeJdLwB2y5lWzVb6-3aLcREnAe5d4uChHQ_h6fmnafX9y9m3V7CVlnnp2W-vYbO_Xso3Bmf19Vu3pf4D8bsq3A |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Colony-stimulating+factor+%28CSF%29+radioimmunoassay%3A+detection+of+a+CSF+subclass+stimulating+macrophage+production&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Stanley%2C+E+R&rft.date=1979-06-01&rft.issn=0027-8424&rft.volume=76&rft.issue=6&rft.spage=2969&rft.epage=2973&rft_id=info:doi/10.1073%2Fpnas.76.6.2969&rft.externalDBID=NO_FULL_TEXT |
thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F76%2F6.cover.gif |
thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F76%2F6.cover.gif |