Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

• Published in: Nature methods Vol. 17; no. 2; pp. 225 - 231
• Main Authors: Zhang, Yongdeng, Schroeder, Lena K., Lessard, Mark D., Kidd, Phylicia, Chung, Jeeyun, Song, Yuanbin, Benedetti, Lorena, Li, Yiming, Ries, Jonas, Grimm, Jonathan B., Lavis, Luke D., De Camilli, Pietro, Rothman, James E., Baddeley, David, Bewersdorf, Joerg
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.02.2020; Nature Publishing Group
• Subjects: 631/1647/245/2225; 631/80/2373/2238; Animals; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Cells; Chemical compounds; Electron microscopy; Endoplasmic reticulum; Fluorescence; Fluorescent indicators; Fluorophores; Golgi apparatus; Humans; Imaging; Life Sciences; Localization; Mammalian cells; Microscopes; Microscopy; Optical Imaging; Organelles; Organelles - metabolism; Proteomics; Subcellular Fractions - metabolism; Switching

Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscop...