Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscop...

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Published inNature methods Vol. 17; no. 2; pp. 225 - 231
Main Authors Zhang, Yongdeng, Schroeder, Lena K., Lessard, Mark D., Kidd, Phylicia, Chung, Jeeyun, Song, Yuanbin, Benedetti, Lorena, Li, Yiming, Ries, Jonas, Grimm, Jonathan B., Lavis, Luke D., De Camilli, Pietro, Rothman, James E., Baddeley, David, Bewersdorf, Joerg
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.02.2020
Nature Publishing Group
Subjects
631/1647/245/2225
631/80/2373/2238
Animals
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Cells
Chemical compounds
Electron microscopy
Endoplasmic reticulum
Fluorescence
Fluorescent indicators
Fluorophores
Golgi apparatus
Humans
Imaging
Life Sciences
Localization
Mammalian cells
Microscopes
Microscopy
Optical Imaging
Organelles
Organelles - metabolism
Proteomics
Subcellular Fractions - metabolism
Switching
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Abstract Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5–10-nm localization precision in three dimensions using ‘salvaged fluorescence’. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes. 4Pi single-molecule switching microscopy combined with ‘salvaged fluorescence’ enables improved ratiometric imaging that bypasses chromatic aberrations and allows for multicolor whole-cell imaging with sub-10-nm localization precision.
AbstractList Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes. 4Pi single-molecule switching microscopy combined with 'salvaged fluorescence' enables improved ratiometric imaging that bypasses chromatic aberrations and allows for multicolor whole-cell imaging with sub-10-nm localization precision.
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5–10-nm localization precision in three dimensions using ‘salvaged fluorescence’. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes. 4Pi single-molecule switching microscopy combined with ‘salvaged fluorescence’ enables improved ratiometric imaging that bypasses chromatic aberrations and allows for multicolor whole-cell imaging with sub-10-nm localization precision.
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.
Audience Academic
Author Bewersdorf, Joerg
Song, Yuanbin
Li, Yiming
Grimm, Jonathan B.
Kidd, Phylicia
Baddeley, David
Lavis, Luke D.
Benedetti, Lorena
De Camilli, Pietro
Rothman, James E.
Lessard, Mark D.
Chung, Jeeyun
Schroeder, Lena K.
Ries, Jonas
Zhang, Yongdeng
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Cites_doi 10.1038/nmeth.1583
10.1371/journal.pone.0142949
10.1073/pnas.1503191112
10.1083/jcb.144.6.1135
10.1038/nmeth.2835
10.1038/nphoton.2016.200
10.1038/nmeth.2488
10.1371/journal.pone.0203291
10.1126/science.1240672
10.1126/science.aab1370
10.1364/OE.16.020774
10.1093/emboj/18.11.2991
10.1038/s41467-018-04220-w
10.1146/annurev-biochem-060815-014801
10.1021/cb800025k
10.1111/boc.201100011
10.1038/nmeth.3528
10.1038/nbt.3876
10.1126/science.aau1044
10.1364/BOE.2.001377
10.1126/science.1247786
10.1364/BOE.10.002708
10.1111/j.0022-2720.2004.01393.x
10.1083/jcb.201809107
10.1038/nbt1107-1234
10.1364/OPTICA.2.000177
10.1364/OE.22.015982
10.1371/journal.pone.0020645
10.1021/nl801471d
10.1038/nmeth.2448
10.1038/nbt765
10.1371/journal.pone.0147506
10.1038/nmeth.1768
10.1007/978-1-4939-7759-8_14
10.1016/j.cell.2013.05.026
10.1073/pnas.0609198104
10.1016/S0092-8674(00)80407-9
10.1038/nmeth.1991
10.1073/pnas.1414814112
10.1073/pnas.0813131106
10.1016/j.cell.2016.06.016
10.1038/nmeth.2472
10.1364/OE.24.028080
ContentType Journal Article
Copyright The Author(s), under exclusive licence to Springer Nature America, Inc. 2020
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References Sigal, Zhou, Zhuang (CR4) 2018; 361
Fernandez-Busnadiego, Saheki, De Camilli (CR27) 2015; 112
Dempsey, Vaughan, Chen, Bates, Zhuang (CR20) 2011; 8
Pallikkuth (CR37) 2018; 13
Li (CR30) 2013; 10
Huang (CR40) 2013; 10
Mlodzianoski, Curthoys, Gunewardene, Carter, Hess (CR19) 2016; 11
Schmitt (CR25) 2007; 104
Keppler (CR33) 2003; 21
Baddeley (CR16) 2011; 6
Van Engelenburg (CR9) 2014; 343
Lampe, Haucke, Sigrist, Heilemann, Schmoranzer (CR17) 2012; 104
Nemoto, De Camilli (CR39) 1999; 18
Chung (CR28) 2015; 349
Giordano (CR29) 2013; 153
Baddeley, Bewersdorf (CR3) 2018; 87
Zhang, Kenny, Hauser, Li, Xu (CR18) 2015; 12
Li, Wu, Hoess, Mund, Ries (CR12) 2019; 10
Bossi (CR15) 2008; 8
Deschamps, Rowald, Ries (CR36) 2016; 24
Wang (CR31) 2014; 22
Takakura (CR34) 2017; 35
Hoess, Mund, Reitberger, Ries (CR42) 2018; 1764
von Middendorff, Egner, Geisler, Hell, Schonle (CR5) 2008; 16
Barr, Puype, Vandekerckhove, Warren (CR26) 1997; 91
Douglass, Sieben, Archetti, Lambert, Manley (CR35) 2016; 10
Ladinsky, Mastronarde, McIntosh, Howell, Staehelin (CR1) 1999; 144
Huang, Schwartz, Byars, Lidke (CR43) 2011; 2
Los (CR32) 2008; 3
Schucker, Holm, Franke, Sauer, Benavente (CR24) 2015; 112
Aquino (CR7) 2011; 8
Burke, Patton, Huang, Bewersdorf, Booth (CR13) 2015; 2
Nieuwenhuizen (CR21) 2013; 10
Ries, Kaplan, Platonova, Eghlidi, Ewers (CR22) 2012; 9
Schonle, Hell (CR14) 2007; 25
Hanser, Gustafsson, Agard, Sedat (CR41) 2004; 216
Jungmann (CR38) 2014; 11
Buttler (CR10) 2018; 9
Shtengel (CR6) 2009; 106
Szymborska (CR23) 2013; 341
Schroeder (CR2) 2019; 218
Carlini, Holden, Douglass, Manley (CR11) 2015; 10
Huang (CR8) 2016; 166
SB Van Engelenburg (676_CR9) 2014; 343
J Deschamps (676_CR36) 2016; 24
A Keppler (676_CR33) 2003; 21
J Chung (676_CR28) 2015; 349
H Takakura (676_CR34) 2017; 35
LK Schroeder (676_CR2) 2019; 218
A Szymborska (676_CR23) 2013; 341
CA Buttler (676_CR10) 2018; 9
K Schucker (676_CR24) 2015; 112
Y Nemoto (676_CR39) 1999; 18
M Bossi (676_CR15) 2008; 8
P Hoess (676_CR42) 2018; 1764
F Giordano (676_CR29) 2013; 153
FA Barr (676_CR26) 1997; 91
C von Middendorff (676_CR5) 2008; 16
J Schmitt (676_CR25) 2007; 104
D Baddeley (676_CR3) 2018; 87
D Baddeley (676_CR16) 2011; 6
R Fernandez-Busnadiego (676_CR27) 2015; 112
F Huang (676_CR40) 2013; 10
RP Nieuwenhuizen (676_CR21) 2013; 10
BM Hanser (676_CR41) 2004; 216
YM Sigal (676_CR4) 2018; 361
L Carlini (676_CR11) 2015; 10
Z Zhang (676_CR18) 2015; 12
MJ Mlodzianoski (676_CR19) 2016; 11
J Ries (676_CR22) 2012; 9
F Huang (676_CR8) 2016; 166
MS Ladinsky (676_CR1) 1999; 144
G Shtengel (676_CR6) 2009; 106
R Jungmann (676_CR38) 2014; 11
D Burke (676_CR13) 2015; 2
Y Wang (676_CR31) 2014; 22
KM Douglass (676_CR35) 2016; 10
GV Los (676_CR32) 2008; 3
YM Li (676_CR12) 2019; 10
X Li (676_CR30) 2013; 10
S Pallikkuth (676_CR37) 2018; 13
A Schonle (676_CR14) 2007; 25
D Aquino (676_CR7) 2011; 8
A Lampe (676_CR17) 2012; 104
GT Dempsey (676_CR20) 2011; 8
F Huang (676_CR43) 2011; 2
References_xml – volume: 216
  start-page: 32
  year: 2004
  end-page: 48
  ident: CR41
  article-title: Phase-retrieved pupil functions in wide-field fluorescence microscopy
  publication-title: J. Microsc.
– volume: 13
  start-page: e0203291
  year: 2018
  ident: CR37
  article-title: Sequential super-resolution imaging using DNA strand displacement
  publication-title: PLoS One
– volume: 18
  start-page: 2991
  year: 1999
  end-page: 3006
  ident: CR39
  article-title: Recruitment of an alternatively spliced form of synaptojanin 2 to mitochondria by the interaction with the PDZ domain of a mitochondrial outer membrane protein
  publication-title: EMBO J.
– volume: 8
  start-page: 2463
  year: 2008
  end-page: 2468
  ident: CR15
  article-title: Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
  publication-title: Nano Lett.
– volume: 10
  start-page: 584
  year: 2013
  end-page: 590
  ident: CR30
  article-title: Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM
  publication-title: Nat. Methods
– volume: 16
  start-page: 20774
  year: 2008
  end-page: 20788
  ident: CR5
  article-title: Isotropic 3D nanoscopy based on single emitter switching
  publication-title: Opt. Express
– volume: 12
  start-page: 935
  year: 2015
  end-page: 938
  ident: CR18
  article-title: Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy
  publication-title: Nat. Methods
– volume: 341
  start-page: 655
  year: 2013
  end-page: 658
  ident: CR23
  article-title: Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging
  publication-title: Science
– volume: 87
  start-page: 965
  year: 2018
  end-page: 989
  ident: CR3
  article-title: Biological insight from super-resolution microscopy: what we can learn from localization-based images
  publication-title: Annu. Rev. Biochem.
– volume: 10
  start-page: 2708
  year: 2019
  end-page: 2718
  ident: CR12
  article-title: Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization
  publication-title: Biomed. Opt. Express
– volume: 8
  start-page: 353
  year: 2011
  end-page: 359
  ident: CR7
  article-title: Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores
  publication-title: Nat. Methods
– volume: 9
  year: 2018
  ident: CR10
  article-title: Single molecule fate of HIV-1 envelope reveals late-stage viral lattice incorporation
  publication-title: Nat. Commun.
– volume: 2
  start-page: 1377
  year: 2011
  end-page: 1393
  ident: CR43
  article-title: Simultaneous multiple-emitter fitting for single molecule super-resolution imaging
  publication-title: Biomed. Opt. Express
– volume: 35
  start-page: 773
  year: 2017
  end-page: 780
  ident: CR34
  article-title: Long time-lapse nanoscopy with spontaneously blinking membrane probes
  publication-title: Nat. Biotechnol.
– volume: 10
  start-page: 557
  year: 2013
  end-page: 562
  ident: CR21
  article-title: Measuring image resolution in optical nanoscopy
  publication-title: Nat. Methods
– volume: 153
  start-page: 1494
  year: 2013
  end-page: 1509
  ident: CR29
  article-title: PI(4,5)P -dependent and Ca -regulated ER-PM interactions mediated by the extended synaptotagmins
  publication-title: Cell
– volume: 3
  start-page: 373
  year: 2008
  end-page: 382
  ident: CR32
  article-title: HaloTag: a novel protein labeling technology for cell imaging and protein analysis
  publication-title: ACS Chem. Biol.
– volume: 10
  start-page: 705
  year: 2016
  end-page: 708
  ident: CR35
  article-title: Super-resolution imaging of multiple cells by optimised flat-field epi-illumination
  publication-title: Nat. Photonics
– volume: 25
  start-page: 1234
  year: 2007
  end-page: 1235
  ident: CR14
  article-title: Fluorescence nanoscopy goes multicolor
  publication-title: Nat. Biotechnol.
– volume: 104
  start-page: 229
  year: 2012
  end-page: 237
  ident: CR17
  article-title: Multi-colour direct STORM with red emitting carbocyanines
  publication-title: Biol. Cell
– volume: 11
  start-page: e0147506
  year: 2016
  ident: CR19
  article-title: Super-resolution imaging of molecular emission spectra and single molecule spectral fluctuations
  publication-title: PLoS One
– volume: 10
  start-page: e0142949
  year: 2015
  ident: CR11
  article-title: Correction of a depth-dependent lateral distortion in 3D super-resolution imaging
  publication-title: PLoS One
– volume: 166
  start-page: 1028
  year: 2016
  end-page: 1040
  ident: CR8
  article-title: Ultra-high resolution 3D imaging of whole cells
  publication-title: Cell
– volume: 349
  start-page: 428
  year: 2015
  end-page: 432
  ident: CR28
  article-title: PI4P/phosphatidylserine countertransport at ORP5- and ORP8-mediated ER–plasma membrane contacts
  publication-title: Science
– volume: 24
  start-page: 28080
  year: 2016
  end-page: 28090
  ident: CR36
  article-title: Efficient homogeneous illumination and optical sectioning for quantitative single-molecule localization microscopy
  publication-title: Opt. Express
– volume: 22
  start-page: 15982
  year: 2014
  end-page: 15991
  ident: CR31
  article-title: Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm
  publication-title: Opt. Express
– volume: 11
  start-page: 313
  year: 2014
  end-page: 318
  ident: CR38
  article-title: Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT
  publication-title: Nat. Methods
– volume: 10
  start-page: 653
  year: 2013
  end-page: 658
  ident: CR40
  article-title: Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms
  publication-title: Nat. Methods
– volume: 112
  start-page: E2004
  year: 2015
  end-page: E2013
  ident: CR27
  article-title: Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum–plasma membrane contact sites
  publication-title: Proc. Natl Acad. Sci. USA
– volume: 1764
  start-page: 237
  year: 2018
  end-page: 251
  ident: CR42
  article-title: Dual-color and 3D super-resolution microscopy of multi-protein assemblies
  publication-title: Methods Mol. Biol.
– volume: 9
  start-page: 582
  year: 2012
  end-page: 584
  ident: CR22
  article-title: A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
  publication-title: Nat. Methods
– volume: 104
  start-page: 7426
  year: 2007
  end-page: 7431
  ident: CR25
  article-title: Transmembrane protein Sun2 is involved in tethering mammalian meiotic telomeres to the nuclear envelope
  publication-title: Proc. Natl Acad. Sci. USA
– volume: 21
  start-page: 86
  year: 2003
  end-page: 89
  ident: CR33
  article-title: A general method for the covalent labeling of fusion proteins with small molecules in vivo
  publication-title: Nat. Biotechnol.
– volume: 361
  start-page: 880
  year: 2018
  end-page: 887
  ident: CR4
  article-title: Visualizing and discovering cellular structures with super-resolution microscopy
  publication-title: Science
– volume: 2
  start-page: 177
  year: 2015
  end-page: 185
  ident: CR13
  article-title: Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy
  publication-title: Optica
– volume: 91
  start-page: 253
  year: 1997
  end-page: 262
  ident: CR26
  article-title: GRASP65, a protein involved in the stacking of Golgi cisternae
  publication-title: Cell
– volume: 8
  start-page: 1027
  year: 2011
  end-page: 1036
  ident: CR20
  article-title: Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging
  publication-title: Nat. Methods
– volume: 6
  start-page: e20645
  year: 2011
  ident: CR16
  article-title: 4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues
  publication-title: PLoS One
– volume: 343
  start-page: 653
  year: 2014
  end-page: 656
  ident: CR9
  article-title: Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits
  publication-title: Science
– volume: 144
  start-page: 1135
  year: 1999
  end-page: 1149
  ident: CR1
  article-title: Golgi structure in three dimensions: functional insights from the normal rat kidney cell
  publication-title: J. Cell Biol.
– volume: 218
  start-page: 83
  year: 2019
  end-page: 96
  ident: CR2
  article-title: Dynamic nanoscale morphology of the ER surveyed by STED microscopy
  publication-title: J. Cell Biol.
– volume: 106
  start-page: 3125
  year: 2009
  end-page: 3130
  ident: CR6
  article-title: Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure
  publication-title: Proc. Natl Acad. Sci. USA
– volume: 112
  start-page: 2029
  year: 2015
  end-page: 2033
  ident: CR24
  article-title: Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution
  publication-title: Proc. Natl Acad. Sci. USA
– volume: 8
  start-page: 353
  year: 2011
  ident: 676_CR7
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1583
– volume: 10
  start-page: e0142949
  year: 2015
  ident: 676_CR11
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0142949
– volume: 112
  start-page: E2004
  year: 2015
  ident: 676_CR27
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1503191112
– volume: 144
  start-page: 1135
  year: 1999
  ident: 676_CR1
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.144.6.1135
– volume: 11
  start-page: 313
  year: 2014
  ident: 676_CR38
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2835
– volume: 10
  start-page: 705
  year: 2016
  ident: 676_CR35
  publication-title: Nat. Photonics
  doi: 10.1038/nphoton.2016.200
– volume: 10
  start-page: 653
  year: 2013
  ident: 676_CR40
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2488
– volume: 13
  start-page: e0203291
  year: 2018
  ident: 676_CR37
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0203291
– volume: 341
  start-page: 655
  year: 2013
  ident: 676_CR23
  publication-title: Science
  doi: 10.1126/science.1240672
– volume: 349
  start-page: 428
  year: 2015
  ident: 676_CR28
  publication-title: Science
  doi: 10.1126/science.aab1370
– volume: 16
  start-page: 20774
  year: 2008
  ident: 676_CR5
  publication-title: Opt. Express
  doi: 10.1364/OE.16.020774
– volume: 18
  start-page: 2991
  year: 1999
  ident: 676_CR39
  publication-title: EMBO J.
  doi: 10.1093/emboj/18.11.2991
– volume: 9
  year: 2018
  ident: 676_CR10
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-018-04220-w
– volume: 87
  start-page: 965
  year: 2018
  ident: 676_CR3
  publication-title: Annu. Rev. Biochem.
  doi: 10.1146/annurev-biochem-060815-014801
– volume: 3
  start-page: 373
  year: 2008
  ident: 676_CR32
  publication-title: ACS Chem. Biol.
  doi: 10.1021/cb800025k
– volume: 104
  start-page: 229
  year: 2012
  ident: 676_CR17
  publication-title: Biol. Cell
  doi: 10.1111/boc.201100011
– volume: 12
  start-page: 935
  year: 2015
  ident: 676_CR18
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3528
– volume: 35
  start-page: 773
  year: 2017
  ident: 676_CR34
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.3876
– volume: 361
  start-page: 880
  year: 2018
  ident: 676_CR4
  publication-title: Science
  doi: 10.1126/science.aau1044
– volume: 2
  start-page: 1377
  year: 2011
  ident: 676_CR43
  publication-title: Biomed. Opt. Express
  doi: 10.1364/BOE.2.001377
– volume: 343
  start-page: 653
  year: 2014
  ident: 676_CR9
  publication-title: Science
  doi: 10.1126/science.1247786
– volume: 10
  start-page: 2708
  year: 2019
  ident: 676_CR12
  publication-title: Biomed. Opt. Express
  doi: 10.1364/BOE.10.002708
– volume: 216
  start-page: 32
  year: 2004
  ident: 676_CR41
  publication-title: J. Microsc.
  doi: 10.1111/j.0022-2720.2004.01393.x
– volume: 218
  start-page: 83
  year: 2019
  ident: 676_CR2
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201809107
– volume: 25
  start-page: 1234
  year: 2007
  ident: 676_CR14
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt1107-1234
– volume: 2
  start-page: 177
  year: 2015
  ident: 676_CR13
  publication-title: Optica
  doi: 10.1364/OPTICA.2.000177
– volume: 22
  start-page: 15982
  year: 2014
  ident: 676_CR31
  publication-title: Opt. Express
  doi: 10.1364/OE.22.015982
– volume: 6
  start-page: e20645
  year: 2011
  ident: 676_CR16
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0020645
– volume: 8
  start-page: 2463
  year: 2008
  ident: 676_CR15
  publication-title: Nano Lett.
  doi: 10.1021/nl801471d
– volume: 10
  start-page: 557
  year: 2013
  ident: 676_CR21
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2448
– volume: 21
  start-page: 86
  year: 2003
  ident: 676_CR33
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt765
– volume: 11
  start-page: e0147506
  year: 2016
  ident: 676_CR19
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0147506
– volume: 8
  start-page: 1027
  year: 2011
  ident: 676_CR20
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1768
– volume: 1764
  start-page: 237
  year: 2018
  ident: 676_CR42
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-4939-7759-8_14
– volume: 153
  start-page: 1494
  year: 2013
  ident: 676_CR29
  publication-title: Cell
  doi: 10.1016/j.cell.2013.05.026
– volume: 104
  start-page: 7426
  year: 2007
  ident: 676_CR25
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.0609198104
– volume: 91
  start-page: 253
  year: 1997
  ident: 676_CR26
  publication-title: Cell
  doi: 10.1016/S0092-8674(00)80407-9
– volume: 9
  start-page: 582
  year: 2012
  ident: 676_CR22
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1991
– volume: 112
  start-page: 2029
  year: 2015
  ident: 676_CR24
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1414814112
– volume: 106
  start-page: 3125
  year: 2009
  ident: 676_CR6
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.0813131106
– volume: 166
  start-page: 1028
  year: 2016
  ident: 676_CR8
  publication-title: Cell
  doi: 10.1016/j.cell.2016.06.016
– volume: 10
  start-page: 584
  year: 2013
  ident: 676_CR30
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2472
– volume: 24
  start-page: 28080
  year: 2016
  ident: 676_CR36
  publication-title: Opt. Express
  doi: 10.1364/OE.24.028080
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Snippet Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial...
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SubjectTerms 631/1647/245/2225
631/80/2373/2238
Animals
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Cells
Chemical compounds
Electron microscopy
Endoplasmic reticulum
Fluorescence
Fluorescent indicators
Fluorophores
Golgi apparatus
Humans
Imaging
Life Sciences
Localization
Mammalian cells
Microscopes
Microscopy
Optical Imaging
Organelles
Organelles - metabolism
Proteomics
Subcellular Fractions - metabolism
Switching
Title Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging
URI https://link.springer.com/article/10.1038/s41592-019-0676-4
https://www.ncbi.nlm.nih.gov/pubmed/31907447
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Volume 17
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