Arabidopsis acetohydroxyacid synthase expressed in Escherichia coli is insensitive to the feedback inhibitors

• Published in: Plant physiology (Bethesda) Vol. 99; no. 3; pp. 812 - 816
• Main Authors: Singh, B. (American Cyanamid Company, Princeton, NJ), Szamosi, I, Hand, J.M, Misra, R
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.07.1992
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Analytical, structural and metabolic biochemistry; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biosynthesis; Cell growth; Chloroplasts; CODE GENETIQUE; CODIGO GENETICO; CRUCIFERAE; Enzymes; Enzymes and enzyme inhibitors; ESCHERICHIA; Escherichia coli; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; HERBICIDAS; HERBICIDE; Herbicides; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; ISOLEUCINA; ISOLEUCINE; LEUCINA; LEUCINE; LIGASAS; LIGASE; Lyases; Molecular Biology and Gene Regulation; Plants; Plasmids; Proteins; Seedlings; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; VALINA; VALINE; Genetic transformation; Enzyme; Escherichia coli; Biosynthesis; Gene expression; Herbicide; Arabidopsis thaliana; Cruciferae; Dicotyledones; Aminoacid; Angiospermae; Acetolactate synthase; Bacteria; Ureas; Spermatophyta; Inhibition; Molecular cloning; Enterobacteriaceae

Acetohydroxyacid synthase (AHAS), the first enzyme unique to the biosynthesis of isoleucine, leucine, and valine, is the target enzyme for several classes of herbicides. The AHAS gene from Arabidopsis thaliana including the chloroplast transit peptide, was cloned into the bacterial expression plasmi...